Expression of MMP-7、c-Jun、c-Fos in Rats Photoaging with DMSCs Transplantation
|School||Zunyi Medical College,|
|Course||Dermatology and Venereology|
|Keywords||MMP-7 c-Jun c-Fos dermal multipotential stem cells skin photoaging|
Objective:This study investigated the relationship of skin photoaging and the expression patten shift of proteins MMP-7, c-Jun, c-Fos in cutaneous of SD rat, in order to provide a theoretical basis for the prevention and treatment of cancer induced by photoaging.Methods:We randomly divided49SD rats into5groups:untreated groups (8rats)and4model groups; photoaging model group(8rats), normal saline group(8rats), nature rover group(8rats) and dermal multipotential stem cells, therapy group(17rats). We treated all SD rats with UVA and UVB ultraviolet light (2h per day, continuous exposure for18weeks)make sure skin photoaging in all rats of model groups,.We randomly kill8rats as the photoaging group; the remaining model rats were randomly divided into normal saline group (8rats)(treated with normal saline), natural recovery group (8rats)(natural recovery) and treatment group (17rats)(treated with1×106dermal multipotential stem cells suspension liquid). We isolated and cutured and identified DMSCs of suckling SD rats then labeled with DAPI. We transplanted the cell sap to the therapy group(9rats) and killed the rats after1d,4d and7d. We tested the survival、migration and settlement of DAPI marked cells in skin photoaging area. Then we treated photoaging skin of the rest rats of therapy group with the cell transplantation for4weeks, and detected the expression of MMP-7, c-jun, c-fos by immunohistochemistry. All the data were analyzed through SPSS17.0statistical software.Results:1. We observed DMSCs Of DAPI marked survive, diffusion, migration at the time point we chose.2. DMSCs could obviously cure skin photoaging.3. Immunohistochemical staining showed:MMP-7expressed in cytoplasma and the expression of MMP-7protein in the photoaging group was higher than that of in rest of groups (P=0.092, Single factor analysis of variance) there was no significant difference among5groups.4. c-Jun and c-Fos expressed at the nuclei of the epidermal cells in the basal layer. The expression of c-Jun and c-Fos protein in the photoaging group was higher than that of in normal skin or treatment group, and there was statistically significant difference among the three groups (P<0.05, sperman related analysis);there was no significant difference among the light group,control group and the negative control group (P>0.05).5. The correlation analysis among indicatored in photoaging skin:a negatively correlation was found between MMP-7and c-Jun, and MMP-7and c-Fos (r=-0.703, r=-0.776, P<0.05); a positive correlation was found between c-Jun and c-Fos in photoaging (r=0.275, P<0.05).Conclusion:The expression abnormalities MMP-7, c-Jun, c-Fos may play an important role in the pathogenesis and development of photoaging; DMSCs may inhibition of oncogene expression in photoaging.