Dissertation > Medicine, health > Clinical > Therapy > Emergency, first aid

Effect of Idocaine on the Expression of High Mobility Group Box1Mrna in Lung of Septic Rats

Author RongFei
Tutor LeiWeiFu; YuJinGui
School Shandong University
Course Anesthesiology
Keywords Lidocaine Sepsis high mobility group box-1(HMGB1) Lung
CLC R459.7
Type Master's thesis
Year 2012
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Objective:To investigate the effect of lidocaine on expression of high mobility group box-?(HMGB1) protein mRNA in lung of septic rats; to explore the mechanism of lidocaine on reducing acute lung injury, which may provide a theoretical basis of lidocaine used for the clinical treatment of sepsis.Methods:50male Wistar rats weighting200~250g were randomly divided into5groups(n=10each):sham operation group(group S), sepsis model group(group L), low, median and high doses of lidocaine groups(LLl,LL2and LL3groups). Each rat had cecal ligation and puncture surgery to establish model of sepsis-induced acute lung injury except those in group S. Each rat in LL1, LL2, LL3groups received intraperitoneal injection with5mg/kg, lOmg/kg, and20mg/kg lidocaine immediately after surgery and at1h,2h postoperative time point, while S and L groups received equal volume of normal saline instead. Rats in each group were randomly sacrificed respectively at24h,48h time point after surgery, and tissue samples from the both lungs were harvested to determine HMGB1mRNA expression by real-time PCR, MPO activity by enzyme-linked immunosorbent assay (ELISA) and the activation of NF-κB by immunohistochemistry. Lung pathology changes were also observed under light microscope.Results:Compared with group S, the HMGB1mRNA expression, MPO activity and cells with NF-κB activation were significantly increased (P<0.05) in the other4groups, and lung tissues have pathological damages in varying degrees. Compared with group L, these indicators in LL1, LL2and LL3groups were decreased(P<0.05).The HMGB1mRNA expression, MPO activity, activation of NF-κB and lung tissue injury changes in LL1,LL2and LL3groups showed a dose-dependent reduction.Conclusion:Lidocaine can attenuate lung injury in septic rats by down-regulating HMGB1gene expression, which shows a dose-dependent effect. The mechanism of the down-regulating effect of lidocaine is related to inhibit the activation of NF-κB.

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