Dissertation
Dissertation > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom )

Study on the Key Factors in the Growth of Mycelium and Sclerotia Formation of Morchella Esculenta

Author ZhuYongZhen
Tutor DuShuangTian;WeiGeHong
School Northwest University of Science and Technology
Course Microbiology
Keywords Morels Nutritional Physiology Sclerotia Environmental factors Growing Media Optimization
CLC S646
Type Master's thesis
Year 2011
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Morel (Morchella esculenta) is not only nutritious, delicious taste, but also has a very high medicinal value to treat a variety of gastrointestinal diseases, improve human immunity, is an urgent need to develop of precious edible and medicinal fungi. The systematic study of this thesis, the morel a nutritional physiological characteristics of the M-4 and main impact of environmental factors on mycelial growth and sclerotia formation in Morchella ternary quadratic general rotary unitized design study based on The training matrix formulations morel sclerotia formed, the main conclusions are as follows: by measuring the growth rate of morel M-4 mycelium in different media, colony diameter and colony growing research different carbon sources , nitrogen sources, inorganic salts, vitamins, and plant growth regulators morel mycelium growth law. The results showed that the: Mycelium growth optimum carbon source for soluble starch; optimum nitrogen source for urea; MnSO 4 (30mg / L) Mycelium growth certain role in promoting; the MgSO 4 K 2 SO 4 , Na the MoO of 2 4 and NaCl the morel mycelium growth is not obvious; the the KH 2 the PO 4 FeSO 4 the ZnSO 4 , Na 2 SeO 3 , CuSO 4 , CoCl 2 and Ni (NO < / sub>) 2 Mycelium growth with significant inhibition. The V B1 V B2 , the V B6 V B12 and folic acid morel mycelium growth no significant effect, niacin (2mg / L) was significantly inhibited the growth of morel mycelium; 6-BA Mycelium growth was not significant, amount to add 2,4-D (≤ 10 mg / L) and indole acetic acid (≤ 2 mg / L) can promote the morel mycelium growth, but higher mass concentration inhibition gibberellin morel mycelium growth inhibition strong. Single factor test to study the different incubation temperature, light intensity, ambient carbon dioxide concentration and matrix initial pH of morel M-4 mycelial growth and sclerotia formation investigated. The results showed that: the morel mycelium optimum growth conditions were as follows: pH 7.7, incubation temperature of 23.6 ° C, light intensity 57.5 lx CO 2 concentration of 2.31%. Morel sclerotia optimum conditions: pH 8.0, temperature 21.7 ° C, light intensity 71.6 lx the CO 2 concentration of 0.59%, moisture content of 56%. 3 single factor test sawdust, corn cob, wheat bran and cotton seed hulls on Morchella sclerotia formation of M-4, on this basis, the ratio of mixing and the general rotation design, by the establishment of a mathematical model study the amount of wood chips, corn cobs, wheat bran and cotton seed hulls morel sclerotia formed the law. The results showed that 17.86% in the sawdust, corn cob 3.57% and 7.14%, wheat bran, cotton seed hulls 71.43% sclerotia formation up.

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