Drought Resistance Identification of New Strain in Transgenetic Wheat by Gm/GhDREB Gene
|School||Northwest University of Science and Technology|
|Course||Crop Genetics and Breeding|
|Keywords||Transgenic Wheat DREB transcription factor Drought Resistance Identification|
Wheat is China's arid and semi-arid areas of major food crops. In increasing water scarcity, droughts increasingly serious harm today, to explore the use of drought-resistant water-saving wheat genetic resources for the improvement of wheat drought resistance of wheat production is important. Transgenic technology development and maturity of short-term cultivation of new varieties of wheat resilience provides a powerful way. In this study, turn GhDREB, GmDREB1 transgenic lines G Lumai 23, G Jimai19 as materials in offspring of genetically modified wheat lines for molecular detection based on simulated drought stress through the indoor and field control treatment, respectively, turn GhDREB, GmDREB1 GM wheat germination stage coleoptile length, radicle number, radicle length and growth in the late relative water content, soluble protein content, relative conductivity, proline, abscisic acid, malondialdehyde content, photosynthetic rate, transpiration rate and other physiological and biochemical changes in agronomic traits, yield analysis for a comprehensive system of comprehensive evaluation of transgenic wheat lines Lumai G 23, G 19 drought Jimai saving performance. An indoor simulation results show that drought stress: seed germination 20% (W / V) PEG-6000 artificial drought stress, the tested material coleoptile length, radicle length and radicle lower than those in non-stress time, by the body control a larger decline, a smaller decline transgenic lines. Two water treatment under control in the late Ota physiological and biochemical parameters were measured results showed that: in the field of water treatment control, with the birth process moves forward, Lumai transgenic G 23, G Jimai19 receptors control plants with chlorophyll content, photosynthetic rate, relative water content have shown a downward trend, but with the receptor compared to control transgenic lines decreased less, but remain at a high level; Lumai transgenic G 23, G Jimai19 soluble protein, proline, abscisic acid content increased with the severity of drought stress continuous accumulation, transgenic lines showed greater; Lumai transgenic G 23, G Jimai19 abscisic acid content is much greater than the control plants, first increased and then decreased trend reaches its maximum at flowering; drought stress conditions in which transgenic plants Lumai G 23, G Jimai19 blades relative conductivity and MDA content increased small amount, indicating transgenic lines Lumai G 23, G 19 can Jimai membrane permeability in leaves and osmotic adjustment and other aspects of physiological regulation itself effectively to accommodate drought stress. 3 agronomic traits yield analysis showed: At W0, W1, W2 three different water treatments, transgenic plant height were always higher than the receptor, with the degree of drought stress enhanced transgenic and control plant height varying degree of reduction, the transgenic plant height amplitude is small; controlled panicles per plant show a downward trend, while the transgenic panicles per plant remained relatively stable; in W0, W1 processing conditions, transgenic grain weight was significantly higher than the control receptor ; the W0 treatment conditions, G Lumai 23 grain yield than the control Lumai 23 receptor yield 11.68%, drought index of 1.22, is a strong drought level, G Jimai19 than receptors control Jimai19 yield 6.36 percent, drought index is 1.23, is a strong drought level, indicating that the receptor transgenic lines were resistant to drought stress than ability.