Dissertation > Medicine, health > Surgery > Surgical operation > Anesthesiology

The Effects of Spinal Cord Transection and Lidocaine Epidural Anesthesia on Propofol Requirement in Rats

Author ZhangNing
Tutor MaHanXiang
School Ningxia Medical University
Course Anesthesiology
Keywords Spinal cord transection Propofol Sedation Epidural anesthesia Lidocaine
CLC R614
Type Master's thesis
Year 2011
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Objective To observe the spinal cord transected rats propofol sedative amount, and to explore its possible mechanism. Methods 45 healthy adult female Sprague-Dawley (SD) rats were randomly divided into three groups: group A: normal spinal cord transection group (n = 15); B: sham group (n = 15); C group: the control group (n = 15). A group of 15 rats received spinal cord transection 5 Excluding experiment, 10 included trials; B group only revealed dura, but does not damage the spinal cord, two Excluding experiment, 13 enrolled in the trial. Group C, 15 rats without any treatment, all included in the trial. Will be selected 38 female SD rats 7 days after the recovery pump by tail vein injection of propofol, the dose of propofol compare three groups of rats eyelid reflex and the time required. Results of the rat spinal cord transection group eyelid reflex disappear when the dose of propofol (6.3 ± 0.9mg/kg) was significantly less than the sham group (11.2 ± 1.1mg/kg) and normal control group (10.8 ± 1.1mg/kg) (P lt; 0.01); the rat eyelid reflection disappears the time required for spinal cord transection group (200 ± 41 s) was significantly less than the sham group (494 ± 46 s) and normal control group (465 ± 51 s) (P lt; 0.01); propofol dosage and time difference of the normal control group and the sham group without statistical significance (P gt; 0.05). Conclusions spinal cord injury can significantly reduce the rat propofol the sedative dosage and shorten the rat eyelid reflexes time. The possible mechanisms of spinal cord injury sedation to incoming theory. Objective To observe the effectiveness of epidural block level in rats propofol sedation, and to explore its possible mechanism. Methods 30 SD rats underwent epidural catheter, 24 was selected. Will be selected for the 24 rats were randomly divided into high-dose lidocaine group (HL group, n = 8), low-dose lidocaine group (LL group, n = 8) and normal saline (NS group, n = 8 ), respectively, in the epidural injection of 2% lidocaine 80 μL, 40μL and saline, 60 μL of a solution; eight SD rats as control group (C group, n = 8). Via the tail vein pump of epidural injection 10min Note C propofol, propofol dosage compare four groups of rats eyelid reflex. HL group and LL group epidural block level was measured using forceps. Results HL groups and LL group eyelid reflex in rats dose of propofol (7.1 ± 0.8) mg · kg -1 and (8.2 ± 1.0) mg · kg - 1 were significantly less than the NS group (10.3 ± 1.1) mg · kg -1 (P lt; 0.01), the HL group and LL group propofol dosage were reduced by approximately 31 % and 20%. NS group and group C (10.4 ± 1.1) mg · kg -1 the propofol dosage differences not statistically significant (P gt; 0.05). The HL group rat eyelid reflex propofol dosage is less than the LL group (P lt; 0.05). HL group and LL group epidural block plane were xiphoid [0.5 (0.5 1 .25)] cm [2.5 (1.75 2 .5) ] cm, the difference was statistically significant (P lt; 0.01). Conclusions lidocaine epidural anesthesia to reduce rat propofol sedative and hypnotic dosage, and the higher the block level, enhanced rat C propofol sedation effect is more obvious.

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