RAS gene polymorphism and Nanjing Han type 2 diabetic dyslipidemia study
|School||Nanjing Normal University|
|Keywords||Angiotensin Ⅰ converting enzyme Of vascular tight prime II type receptor Angiotensin Ⅰ converting enzyme 2 Renin - angiotensin system Gene polymorphism Type 2 diabetes mellitus Dyslipidemia|
Objective: renin - angiotensin system (Renin-Angiotensin System, RAS) gene polymorphism has been reported associated with cardiovascular disease. However, the RAS polymorphisms with type 2 diabetes blood lipid abnormalities (type 2 diabetes, T2D) research at home and abroad are rare. Therefore, the purpose of this study is to investigate the angiotensin-converting enzyme (angiotensin-converting I enzyme, ACE) gene insertion / deletion (insertion / deletion, I / D) polymorphism, angiotensin Ⅱ 1 receptor (angiotensin II type 1 receptor, AT1R) (rs5186) and angiotensin-converting enzyme 2 (angiotensinconverting enzyme 2, ACE2) (rs2285666) relationship Nanjing Han patients with type 2 diabetes, abnormal blood lipids. Methods: We conducted a case - controlled trials, 788 cases of study from Nanjing, including 282 cases of diabetic dyslipidemia group (A), 182 cases of diabetes with normal lipid group (B), and 324 healthy control group. Respectively, using the polymerase chain reaction (polymerase chain reaction, PCR) and PCR-restriction fragment length polymorphism (PCR-restriction fragment length polymorphism, PCR-RFLP) ACE (I / D), AT1R (rs5186) and ACE2 (rs2285666) polymorphism detection. By specific polymorphisms with which a group of risk assessment rate (odds ratio, OR). Results: The comparison of the A group and the control group, ACE (I / D) D allele with T2D blood lipid abnormality was significantly related (OR = 1.37, 95% CI = 1.08-1.74; P = 0.010); Group A comparison of group B, the D allele with lipid abnormalities significantly related (OR = 1.88,95% CI = 1.40-2.54; P lt; 0.001); comparison of the B group and the control group, the ID genotype with T2D lipids The normal risk reduced significantly associated (OR = 0.52,95% CI = 0.32-0.82; P = 0.0060). Among the three groups ATlR (rs5186) and ACE2 (rs2285666) genotype and allele frequencies were not statistically significant. Joint analysis of three genetic polymorphisms of T2D patients in the ACE gene ID / DD AT1R gene AC type dyslipidemia was significantly associated (OR = 2.13,95% CI = I.39-3.26; P lt; 0.001) the ACE gene ID / DD AT1R gene GA / AA type of dyslipidemia was significantly associated (OR = 12.13,95% CI = 2.55-57.69; P = 0.0017). Of T2D group ACE genotypes between ACE activity significant differences, ID and DD genotype of ACE activity in group A than in group B Conclusion: This study showed that the ACE (I / D) polymorphism with the Nanjing area the Han population T2D dyslipidemia or normal occurrence on the DD genotype and D allele may T2D patients who suffer from dyslipidemia predisposing factors. Not found AT1R (rs5186) and ACE2 (rs2285666) polymorphism single factor T2D dyslipidemia or related to normal. Through the joint analysis of ACE and of AT1R, the ACE and ACE2 gene polymorphism in T2D dyslipidemia synergies that may exist. The T2D patients dyslipidemia may be related to ACE activity related to the rise.