Effect of Different Post-processing Yeast Selenium on Antioxidation, Immunity and Se-retention of Mice
|School||Wuhan Polytechnic University|
|Course||Food Science and Engineering|
|Keywords||High selenium yeast After-treatment Antioxidant Immunity Retentive|
The purpose of this project is to use a different process made of the different forms of selenium-enriched yeast, selenium yeast in a variety of post-processing through a series of animal studies to play in the animal body of biological effects and functional activity of yeast contained selenium elements in the lifetime of the animals, provide strong data support for the selection of the best high selenium yeast process. Test using male Kunming mice as test animals, in mixed cages and periodically change the placement of the cage to reduce the breeding environment mouse growth caused by the impact. Administered by gavage, the gavaged volume is adjusted periodically according to the body weight of mice. By electron microscopy can clearly understand, microscopic photos of different post-processing of high selenium yeast selenium yeast in yeast cell morphology changes before and after treatment, and treatment. Selenium yeast in selenium retention in vivo test to study the selenium content the 7d in mouse liver after stopping. The results show that: the inorganic selenium sodium selenite group after stopping the selenium content in the fastest decline; while high selenium yeast autolysis slowest decline and withdrawal remained high content of selenium in the livers of mice at 7d ( P lt; 0.01). The main function of physiological activity in selenium antioxidant effects. Antioxidant activity of selenium-enriched yeast performance is better than the other post-processing group (P lt; 0.05), high selenium yeast autolysis group on indicators of liver selenium content in addition to antioxidant selenium content test results show that: ultrafine grinding-high selenium yeast group, significantly better than the other post-processing group (P lt; 0.01). Immune indicators, the test results of individual mice in each experimental group are quite different, the impact of the effect of high selenium yeast group comparison. The lysozyme content indicators ultrafine grinding effect of selenium yeast group (P lt; 0.05), and other indicators, such as organ index, delayed type hypersensitivity and anti-serum hemagglutination volume number showed no significant difference ( P gt; 0.05). Compared to the tested mice fed moldy corn mixed with feed, play of different post-processing of the biological function of the high selenium yeast. Antioxidant activity of selenium-enriched yeast group on GSH-Px best performance, which is consistent with previous test results, but with the difference between the other groups was not statistically significant (P gt; 0.05), ultrafine grinding rich selenium yeast group T-SOD indicators is better than the other groups (P lt; 0.05); immune aspects, in addition to ultra-fine powder high selenium yeast in serum hemagglutination set indicators better than the other groups (P lt; 0.05 ), the other two indicators for each post-processing between high selenium yeast group and the blank group difference was not statistically significant (P gt; 0.05); liver selenium content, high selenium yeast autolysis group is still better than the other groups (P lt ; 0.05). Can be seen through a series of tests, different post-processing of high selenium yeast have focused on the effectiveness of each to play in the animal body, antioxidant activity and immune activity to improve the animal's body, the activity of selenium-enriched yeast and ultrafine grinding selenium-enriched yeast better than dusting selenium-enriched yeast and selenium-enriched yeast, combined with the production costs of enterprises autolysis activity of selenium-enriched yeast is a better choice. If you take into account the development of products as livestock feed selenium supplements to increase the selenium content in the animal body tissues, high selenium yeast autolysis process should choose.