Breeding Technology of Paeonia Suffruticosa and Analysis of Genetic Diversity of F1Progenies of Paeonia Suffuruticosa
|School||Shandong Agricultural University|
|Course||Ornamental Plants and Horticulture|
|Keywords||Paeonia breeding technique F1progenies SRAP mixed-pollen cross|
Paeonia suffruticosa was sheepberry and be as the medicine in the early stage. It is oneof ten famous Precious flowers, and has longer than2000years cultivation history in China.In this study, we aimed to breed new varieties of Paeonia that according to some specialneeds by radioactive breeding and cross breeding. Sequence related amplified polymorphism（SRAP）was conducted to assess the genetic diversity among the32F1progenies and toanalyze the genetic affinity between F1progenies and parental generation of Paeoniasuffruticosa from a mixed-pollen cross(every kind of pollen was mixed equally) and took5pollen parents as control, which provide scientific foundation for seed selection and breedingof Paeonia suffruticosa in future.The main results were as follows:1. This experimentation adopted hybridization by hand pollination and establishedhybridized combination by the special breeding destination. The setting percentage of75hybridized combinations was more than70%.2. The effects of radioactive breeding to the germination percentage of seed, the rate ofemergence, the seeding height and the root length.2.1The germination percentage and the emergence rate of the seed was radiated bydifferent radiation had different degree effects. The germination percentagehe was the largestwhen be radiated by17.46Gy, between17.46Gy to43.65Gy had not clear difference than CK,and more than43.65Gy was declined. The emergence rate was the largest when be radiated by17.46Gy, but was not markeness than CK, which showd that the dose of60Co-γ radiation inreason can accelerate emergence.2.2The seedings height that be radiated by different radiation were increased than CK ondifferent degrees and the seedings height was radiated by17.46Gy competed with CK havingmarkeness difference, which showed60Co-γ radiation had clear auxo-action to seedlinggrowth of Paeonia.2.3The root length on different60Co-γ radiation dose was higher than collation, but thedifference was not markedness.3. Analysis of Genetic Diversity of F1progenies of Paeonia suffuruticosa by SRAP3.1Orthogonal experimental design was conducted to establish the reaction system for SRAP-PCR of F1progenies of Paeonia. The total25μl reaction volume including2.5μl of10×PCR buffer,2.0mmol/L of Mg2+,0.25mmol/L of dNTPs,1.5U of Taq enzyme,0.25μmol/L of each primer,2.0ng/μL of DNAtemplate. The influences of each factor on thePCR-reaction system is Taq enzyme>dNTPs>primers>Mg2+>DNA template. Amplificationprogram was:denatured for5min at94℃; the primer5reaction cycles were1min at94℃,1min at35℃,1min at72℃; the following35cycles were1min at94℃,1min at50℃,1min at72℃;then10min at72℃and stored at4℃.3.225primer pairs were selected from100pairs, which were applied to study thegenetic diversity and relationships of the materials.25primer pairs produced305loci ofwhich300were polymorphic, the percentage of polymorphic loci was98.36%, each primercombination produced12.2loci and12polymorphic loci; the effective number of allele was1.9046~1.4354with an average of1.6998; the Nei’s genetic diversity (H) was0.4725~0.2759with an average of0.3938; and the Shannon’s information index (I) was0.6649~0.4340withan average of0.5743.3.3The Nei’s genetic similarity coefficient (GS) ranged from0.4590to0.7541with anaverage of0.6287, showed that the genetic relationship of the materials was near. The geneticsimilarity coefficient between ‘Z4’ and ‘Zhushalei’ was0.4590and the genetic distance was0.7787, which were the farthest; the genetic similarity coefficient between ‘Z7’ and ‘Z9’ was0.7541and the genetic distance was0.2822.3.4The32F1progenies of Paeonia suffuruticosa of the average of the Nei’s genetic diversity(H) was0.3941and an average of the Shannon’s information index (I) was0.5740. The5check varieties of an average of the Nei’s genetic diversity (H) was0.3144and the average ofthe Shannon’s information index (I) was0.4598. The results showed that the F1progenies ofPaeonia suffuruticosa have more distinct variation and the genetic diversity of the F1progenies of Paeonia suffuruticosa is more abundant.3.5A dendrogram was constructed based on SRAP data using UPGMA cluster method.The32F1progenies of Paeonia suffruticosa were classified into4major groups，which wasbasically corresponded with the genetic relationships based on color traits. And the femaleparents have the similar color, whose F1progenies have the near genetic relationships.3.6Compared with flower types, plant types and leaf types of F1progenies, the colorheredity of female parent has more stable.