Dissertation
Dissertation > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Wildlife Diseases > Animals used for experiments

The Protective of Vitamin C and Vitamin E for Reproductive Damage of Mo-exposed Female Mice

Author ChenXiaoLi
Tutor YuXueLi
School Henan University of Science and Technology
Course Animal Genetic Breeding and Reproduction
Keywords Molybdenum Embryo Oocyte Vitamin Oxidative stress Mouse
CLC S858.91
Type Master's thesis
Year 2013
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Objective:Molybdenum is widely found in nature, but it is also essential traceelements in normal life activities of the animal body. Excessive intake of molybdenum(Mo), will lead to tissue damages and functional changes. In this study, byconstructing reproductive damage model of female mice exposed to differentconcentration of molybdenum to observe the effects of molybdenum on the ovariantissue oocytes, formulated vitamins C and E’s protection mechanism to Mo exposedmice with reproductive function injury, and provided reference frame for thetreatment of Mo exposed mice with reproductive function damage.Methods:1. The effect of Mo on mouse preimplantation embryo development invitro: Zygotes were flushed from one outbred mouse strain (Kunming), and then werecultured in potassium simplex optimized medium (KSOM) containing0,5,10,20,40,80,120, and160μg/mL of molybdenum for5days until the mid-blastocyst stage. Theaddition of≤20μg/mL molybdenum did not affect the blastocyst and birth rates.2.Molybdenum influence on ovarian function in mice: ICR strains of adult female micewere divided into five groups, exposed through drinking water, the concentration ofmolybdenum in the drinking water for0,5,10,20,40μg/mL. After14days, harvetingthe mouse MII oocytes and ovarian tissue, MII of the total number of oocytes andmalformation rate, ovarian index, ultrastructural changes of ovary,the ovarianantioxidant enzymes (superoxide dismutase) and glutathione peroxidase activity andlipid peroxidation the malondialdehyde level to assess the impact of molybdenum onovarian function.3. Distribution of metal concentrations in ovary and the whole blood:The blood and ovaries were collected and measured the content of molybdenum,copper, ferrum, zinc, and manganum in the0,20, and40μg/mL group.4. Thetreatment of Vitamin C, E to reproductive toxicity by Mo in female mice: Theexperiment was divided into five groups namely Water+Oil (negative control group),the Mo+Oil (positive control group), Mo+VC+Oil group (VC group), Mo+VE+Oil (VE group), Mo+VC+VE+Oil (VC+VE group). Molybdenum exposures throughdrinking water in14days, concentrations were40μg/mL, with vitamin C and/orvitamin E treatment for7days. Mouse MII oocytes and ovaries were collected, themorphology of them observed, records related to ovarian parameters analyzed and thebiochemical indicator of ovary detected, for the assessment of therapeutic effect ofvitamin C, E.Result:1. Mo’s effect to mouse preimplantation embryo development in vitro: whenthe molybdenum concentration≤10μg/mL, molybdenum did not significantly affectthe quality of the embryos. The addition of≤20μg/mL molybdenum did not affect theblastocyst and birth rates. Molybdenum at doses of40μg/mL and higher significantlydecreased the cleavage, blastocyst and birth rates, the average cell number, andsignificantly increased the proportion of degenerative blastocysts.2. Molybdenuminfluence on ovarian function in mice: Compared to control, the MII oocytemorphology, ovary index, ovulation were improved by Mo at5μg/mL, but werenegatively affected by Mo at40μg/mL. When the molybdenum concentration≥20μg/mL, ovarian oocytes and granulosa cells, mitochondria had a certain degree ofdamage. Oocyte cytoplasm containing a relatively small number of cells, as well assmaller, abnormal morphology, mitochondrial swelling and deformation ridge. Allthese alterations were accompanied with the changes in SOD, GPx and MDA levels inovaries.3. Distribution of metal concentrations in ovary and the whole blood: Thecontent of Mo was extremely increased in the whole blood and ovaries, in the sametime, the Zn、Fe、Mn contents were extremely decreased. Mo at≥40μg/mL markedlydecreased the contents of Cu、 Zn、 Mn.4. Vitamin C, E abirritated femalereproductive toxicity in mice: mice exposed in the40μg/mL molybdenum, respectively,with the right amount of VC, VE and VC and VE by oral treatment, found a protectiveeffect to mice ovarian oxidative damage.Conclution:1.molybdenum negatively affected the development of embryos in adose-dependent manner. With lower doses (≤20μg/mL), mouse embryos were notapparently damaged. With very high doses (≥40μg/mL), embryo quality significantlydecreased. This assessment of the effect of molybdenum on the preimplantationembryo is an initial survey of toxicological risk.2. Mo affects the oocyte qualitythrough regulating the ovarian oxidative stress in a dose-dependent manner. Especially,the Mo improves ovarian functions at a suitable concentration, which might be a novel drug candidate for the treatment of female infertility.3. Molybdenum can beaccumulated in ovaries in mice, and have the antagonistic relationship between withZn, Fe, and Mn. When Mo at≥40μg/mL, the shortage of Cu, Zn, and Mn resulted inthe decrease of activity of Cu-Zn SOD and Mn SOD.4. VC, VE has a role in abatingmouse ovarian oxidative damage caused by molybdenum; VC and VE combined effecthas a better protective effect to ovaries in mice.

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