Dissertation
Dissertation > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Wildlife Diseases > Animals used for experiments

Preparation of Chito-oligosaccharides Chelating Vanadium and Comparative Study of Its Hypoglycemic Function

Author WeiDan
Tutor WangXiuWu
School Liaoning Normal University
Course Zoology
Keywords chito-oligosaccharides chito-oligosaccharides chelating vanadium mice bloodsugar
CLC S858.91
Type Master's thesis
Year 2012
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Vanadium has an important role of the organism growth,development and metabolism. Beginning in the1980s, inorganic vanadium compounds had been studied in depth, and it has a therapeutic effect on diabetes.In the United States it has even been used in clinical, but due to some shortcomings of its own, it limits the development.Scientists began to explore the hypoglycemic function of organic vanadium compounds.Chito-oligosaccharides (Chito-oligosaccharides, COS)which is degradated by chitosan,is also known as glucosamine oligosaccharide, its absorption and the utilization rate are high, and the water solubility is good, in terms of physiological function the spleen antibody formation promotes and the liver features are enhanced.In this thesis, chito-oligosaccharides(average molecular weight1000)and sodium orthovanadate(Na3VO4·12H2O)as raw materials, prepare the chito-oligosaccharides chelating vanadium by controlling reaction mass ratio, reaction time, temperature and pH. The highest yield of chito-oligosaccharides chelating vanadium is28.11%, vanadate may occur with the chito-oligosaccharides molecules of amino and hydroxyl by infrared spectroscopy analysis. Order to investigate the hypoglycemic function of the chito-oligosaccharides chelating vanadium, conducting the Kunming male mice as the animal experiments to study the hypoglycemic function of the the chito-oligosaccharides chelating vanadium on diabetic mice and damage and repair of the liver, kidney and spleen. Add0.7g/kg of chito-oligosaccharides and chito-oligosaccharides chelating vanadium in feed, through the four weeks, the results show that the weight of the diabetic control group decreased gradually after the experiment start,when the experiment ends, the weight of mice decreased by1.91g, with the normal control group decreased by29.69%. When the experiment starts, in the first two weeks the weight of the chito-oligosaccharides group and the chito-oligosaccharides chelating vanadium group is decreased, from the third week the weight gain, compared with the diabetic control group when the experiment ends it increased by17.57%and27.99%, and the difference is significant(P<0.05). Food intake and water intake results show that food intake and water intake in the diabetic group with an upward trend from the second week began to decline, at the end of the experiment, compared with the diabetes control group(32.44g), the chito-oligosaccharides group(19.62g)reduced by39.52%,and compared with the diabetes control group(32.44g), the chito-oligosaccharides chelating vanadium group(18.85g) is lower by41.89%,and the difference is significant(P<0.05), but the chito-oligosaccharides group and chito-oligosaccharides chelating vanadium group have no differences significantly(P>0.05). In blood sugar, the blood sugar of the diabetic control group has been increasing, at the end of the experiment, compared with the normal control group, it has the significantly difference(P<0.05). While the blood sugar of the the chito-oligosaccharides group(15.59mmol/L) at the end of the experiment is lower than the diabetes control group(19.03mmol/L)by18.08%, it also has a significantly difference(P<0.05); compared with diabetes control group (19.03mmol/L)the blood sugar of chito-oligosaccharides chelating vanadium group(15.06mmol/L) at the end of the experiment is decreased by20.86%, however,there are no significantly differences(P>0.05). From the liver, kidney and spleen morphology experiments we also find that the liver, kidney and spleen of the diabetic control group are swelling, color deepen and they are seriously injuried, while the damnification of liver, kidney and spleen in chito-oligosaccharides group and chito-oligosaccharides chelating vanadium group reduce and the effect of repairiment is obvious.

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