Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification

Studies on Chemical Constituents of Stemona Tuberosa Lour

Author YueYong
Tutor QinHaiLin
School Beijing Union Medical College
Course Medicinal Chemistry
Keywords Stemona tuberose chemical constituents stemona alkaloids acetylcholinesterase inhibition
CLC R284.1
Type PhD thesis
Year 2013
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The herb of Radix Stemonae (’Baibu’in Chinese) has been widely used as an antitussive and insecticide remedy for thousands of years in China. Modern pharmacological studies suggest that Baibu has a good insecticidal activity; and it also has a central nervous system sedative and analgesic effects. The species Stemona tuberose Lour, which distributs mainly in ShanXi, ShangDong, AnHui, and JiangSu Provinces and so on, is documented in China Pharmacopoeia as one of three original plant resources for Baibu. Until now, there have been more than sixty stemona alkaloids belonging to different types being isolated from the Stemona tuberosa. In order to get more new stemona alkaloids and to study their structure-activity relationship, this dissertation has conducted the systemic investigation on the chemical constituents of Stemona tuberosa collected from Enshi, Hubei province. Various chromatographic methods were used and twenty-nine compounds were obtained from the95%ethyl alcohol extract of the radix, and their structures, including the relative configuration, were identified according to the physical and chemical data and the spectroscopic characteristics. These compounds are as follows: Ineotuberostemonol(1), tuberostemonine L(2), tuberostemonine M(3), tuberostemonoxirine(4),9a-epi-tuberospironine(5), tuberospironine A(6), tuberostemonoxonine(7), Neotuberostemonone D(8) Neotuberostemonone A(9), Neotuberostemonone5(10), Neotuberostemonone C(11), Neotuberostemononol A(12), Neotuberostemononol B(13), Sessilifoliamide11(14), la-tuberastemoninol(15), stilbostemin Z(16),4-methoxy-l-methyl-2,7,-dihydroxy-9,10,-dihydrophenanthrene (17), tuberostemoline(18),β-sitosterol(19), stigmasterol(20), β-daucosterol(21), tuberostemonone(22), didehydrotuberostemonine (23),9a-bisdehydrotuberostemonine(24), Sessilifoliamide F(25), sessilifoliamide G(26),2’-Hydroxyethyl-4-hydroxybenzoate(27),3-O-Caffeoyl-y-quinide(28),2,5-Furandimethanol(29). Of which, compounds1-15are new stemona alkaloids, compounds16and17are two new stilbenoids. There are totally twenty-one stemona alkaloids, with compounds1-4,7,23, and24belonging to Stenine alkaloid, compounds8-15,18,22,25, and26being Miscellaneous alkaloid, and compounds5and6belonging to the Tuberostemospironine alkaloid (classification see review). Additionally, compounds27-29are isolated from this plant for the first time.14alkaloids have been carried on the acetylcholinesterase (AchE) inhibition experiment, the compound1,3have displayed weak bioactivity, in5u g/ml examination density, its inhibition rate respectively is11.26%,22.83%, other compound have not seen the obvious bioactivity.

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