Dissertation > Medicine, health > Medical research methods > Experimental medicine, medical experiments > Medical Laboratory Animal Science

The Influence of Saccharomyces Cerevisiae Boulardii on Rats Model of Cirrhosis

Author ZhuLin
Tutor YuanJieLi
School Dalian Medical University
Course Microbiology
Keywords Saccharomyces boulardii Cirrhosis Rats Endotoxemia Intestinal flora
CLC R-332
Type Master's thesis
Year 2013
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Purpose:Cirrhosis clinical common chronic progressive liver disease, and is formed by oneor more of the cause of long-term or repeated diffuse liver injury. Impaired liverfunction, bile secretion through the intestinal liver axis to change, reducied the bloodsupply and intestinal peristalsis and other factors, led to the destruction of the intestinalmucosa and intestinal flora imbalance[1`2]. The changes the intestinal flora to furtherpromote severe liver injury complications, such as bacterial translocation andendotoxin-induced secondary bacterial infection and sepsis, is one of the main cause ofdeath in patients. The presence of gut microflora imbalance in patients with liverdisease, the extent and the severity of liver disease[3].This experiment is givenSaccharomyces boulardii cirrhotic rats induced by carbon tetrachloride treatment, toobserve the role of liver cirrhosis in rats provide a theoretical basis for the preventionand treatment of cirrhosis of the liver.Methods:Fellow controls Wistar rats,50wistar rats were divided into normal group (Zgroup), model group (M), the a probiotic prevention group (W group) and treatmentgroup (Y group). Be multiple sites subcutaneous injection of50%carbon tetrachloridesalad oil solution, M, Y, W group to0.5ml/100g dose, injection2times per week andtheir body weight was weighed, As its sole source of drinking water to the10%ethanolsolution (prepared food liquor add distilled), molding cycle for14weeks. W groupgavage once each time orally, Saccharomyces boulardii formulations,0.5×108until theend of the experiment. After the end of the molding, Y group should daily gavage once,Saccharomyces boulardii formulations,0.5×108. Time4Weeks. Normal group andmodel group rats were given the same saline. During the experiment observed in thenormal group, model group and prevent rats coat, mental status, and living habits. The end of the experiment, checking serum ALT, AST, ALB, MDA and liver functionindicators, detect plasma endotoxin levels, By DGGE-PCR to detect intestinalmicroflora.Results:Endotoxin in the plasma of rats of the model group was significantly higher thanthe normal group, the prevention and treatment groups. Prevention group and normalrats differentiated plasma endotoxin content, but not statistically significant. Normalintestinal flora rats significantly better than the model group, liver cirrhosis rat intestinalflora imbalance by denaturing gradient gel electrophoresis. Treatment group rangedbetween prevention and model groups. Prove molding Saccharomyces boulardii givenafter gavage, has played a significant role in the regulation of intestinal flora.Conclusion:Saccharomyces boulardii improving cirrhosis model rat intestinal flora to reducethe significance of intestinal endotoxemia hyperlipidemia

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