Dissertation > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Abnormal blood pressure > Hypertension

Association Study of the β1-adrenoceptor Gene Single-nucleotide Polymorphisms and Essential Hypertension in a Northern Han Chinese Population

Author WangZuo
Tutor WenShaoJun
School Capital University of Medical Sciences
Course Internal Medicine
Keywords Receptor adrenergic beta-1 Hypertension Polymorphism genetic Case-Control Study Genetic Predisposition to Disease the Northern Han Chinese
CLC R544.1
Type Master's thesis
Year 2013
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Objective This study was to systematically explore the possible association betweenthe Arg389Gly and Ser49Gly single-nucleotide polymorphisms (SNPs) in the-adrenoceptor (ADRB1) gene and essential hypertension (EH) risk among theNorthern Han Chinese population. The association between the genotypes andhaplotypes of the SNPs and EH risk was researched in the current study. Thiscase-control study was to provide evidence for the prevention and treatment of EH.Methodology This study included712hypertensive subjects and663healthyvolunteers as control subjects in the Northern Han Chinese. Genotyping wasperformed to identify the Arg389Gly and Ser49Gly polymorphisms of the ADRB1gene. In this case-control study in the Northern Han Chinese population, the Gly389allele frequency of Arg389Gly was0.25for the EH group and0.26for the contro lgroup. And the Gly49allele frequency of Ser49Gly was0.14for the EH group and0.16for the control group. The association between these polymorphisms andessential hypertension in the entire sample population was not significant. And nosignificant association has been found for the two polymorphisms in all geneticmodels (including additive, dominant, recessive and homozygote comparisons). Inthe stratified analysis performed by gender, still no positive association has beenobserved. In addition, no linkage disequilibrium was tested between the Arg389Glyand Ser49Gly polymorphisms in current study.Results1Univariate analyses indicated that the genotype distribution and allelic frequency ofthe Arg389Gly and Ser49Gly polymorphisms were similar between the case groupand the control group. Logistic regression analysis was performed after adjusting forconfounding risk variables. For Arg389Gly polymorphim, still no positive association was found in all the genetic models: in allelic comparison {Gly vs. Arg OR=0.89795%CI [0.6341.270] P=0.541} in dominant genetic model {GlyGly+ArgGlyvs. ArgArg OR=0.97895%CI [0.6301.519] P=0.923} in ressesive genetic model{GlyGly vs. ArgGly+ArgArg OR=0.61195%CI [0.2761.349] P=0.223}in homozygote comparison {GlyGly vs. ArgArg OR=1.76895%CI [0.7614.111]P=0.185} and in additive genetic model {GlyGly vs. ArgGly vs. ArgArg OR=0.90295%CI [0.6431.266] P=0.551}. For Ser49Gly polymorphim, we also found nosignificant association in all the genetic models: in allelic comparison {Gly vs. SerOR=0.83395%CI [0.5861.183] P=0.307} in dominant genetic model{GlyGly+SerGly vs. SerSer OR=0.79095%CI [0.5301.177] P=0.247}in ressesive genetic model {GlyGly vs. SerGly+SerSer OR=1.00595%CI [0.2673.788] P=0.994} in homozygote comparison {GlyGly vs. SerSer OR=0.97095%CI[0.2533.726] P=0.965} and in additive genetic model {GlyGly vs. SerGly vs.SerSer OR=0.82695%CI [0.5771.183] P=0.298}.2We also performed a case-control study using a much more strict criteria forhypertension (SBP160mmHg and/or diastolic blood pressure90mmHg).Nevertheless, still no positive associations were gained for the two polymorphismswith EH risk (Arg389Gly Pgenotype=0.622Pallele=0.547Ser49Gly Pgenotype=0.239Pallele=0.118).3Subgroup analyses were performed by gender and no significant result was foundin both males and females.4The ADRB1gene Arg389Gly and Ser49Gly polymorphisms were not in linkagedisequilibrium (LD).Conclusions1The current case-control study suggested that ADRB1Arg389Gly and Ser49Glypolymorphisms might not be related to the increased risk of essential hypertension in the Northern Han Chinese population.2The Arg389Gly and Ser49Gly polymorphisms might not be in linkagedisequilibrium.

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