Dissertation
Dissertation > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Respiratory failure

Study about Intervention of Radix Salviae Miltiorrhizae on Angiogenin(ang) in Rats with Acute Lung Injury

Author LeiZuo
Tutor ZuoLiLi
School Chongqing Medical University
Course Internal Medicine
Keywords acute lung injury angiotensin-1 angiotensin-2 radixsalviae miltiorrhizae
CLC R563.8
Type Master's thesis
Year 2013
Downloads 13
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Objective: explore the possible involvement of ang-1and ang-2inlipopolysaccharide-induced rat pulmonary inflammatory response in acutelung injury and study on the mechanism of action and interventionmechanism of radix salviae miltiorrhizae on it. Method: we selected36maleSD rats (clean conventional rats),then have built three groups.①Normalcontrol group12rats: the group was given the same amount of normal salinefrom the tail vein;②LPS group12rats: the group was given the LPS5mg/kg from the tail vein;③radix salviae miltiorrhizae group12rats:thegroup was given the LPS5mg/kg from the tail vein,one hours later the groupwas received injection of Salvia miltiorrhiza10g/kg(zheng da qingchunbaolimited production10g)。after12hours,every rat was killed.the BALF、bleed、lung tissue have been collected to examine the W/D、LI、LPI and theexpression of Ang-1、Ang-2in lung by western-blot。Results:the W/D、LI、LPI of LPS and salviae groups is higher than those of NS group(p<0.01),thegradingofpathologicdamageoflungtissuewasobvious.the expression of ang-1in lung by NS group which is higher than that in LPSand salviae groups(p<0.05).ang-2expression were significantly higher inLPS and salviae group than NS group (p<0.01).compared with salviaegroup, the ang-2expression、the ratio of wet/dry weight、LI and LPI in LPSgroup were significantly increased(p<0.05). Conclusion:the ratio of ang-1and ang-2expression play an important role in the stability of pulmonaryvascular endothelial cell,which happens during acute lung injury. the salviaecould down-regulate ang-2expression and up-regulate ang-1expression inALI rats,lung tissue to reduce the inflammation of alveolar space.it could theless red cell diapedesis,but the mechanism is understand.

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