Dissertation
Dissertation > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology

Effect of Thiazolidinediones on Atherosclerosis of Diabetic Rats

Author WangWeiJing
Tutor GaoDongLai
School Shanxi Medical
Course Department of Cardiology
Keywords atherosclerosis diabetes thiazolidinediones osteogrotegerin
CLC R965
Type Master's thesis
Year 2013
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Objective:To investigate the effect of thiazolidinediones on atherosclerosis (AS) of diabetic rats, and the expression of osteogrotegerin in atherosclerosis of diabetic rats. Methods Respectively to build a rat model of atherosclerosis for warfarin plus vitamin D3,a diabetes (DM) model for chain urine with cephalosporins.Methods:Take36male SD rats and randomly divided into6groups on average: AS group, DM group, AS+DM group, control group, AS+TZD group, AS+DM+TZD group. Measurements of metabolic parameters, von Kossa calcium dyeing, aortic calcium density and ALP (alkaline phosphatase) liveness, qRT-PCR for OPG (osteoprotegerin) mRNA.and immunohistochemistry for OPG were performed. ResultsResults:In AS group and AS+DM group,calcium deposition in aorta;aortic ALP liveness and calcium density of AS+DM group respectively rise3.70times,1.30times and3.64times,1.35times compared to AS group and DM group(P<0.05), OPG (osteoprotegerin) mRNA and expression decreased (P<0.05). Aortic ALP liveness and calcium density of AS+DM+TZD group were reduced18.03%and13.69%compared to AS+DM group(P<0.05), OPG (osteoprotegerin) mRNA and expression increased(P<0.05).Conclusion:Long-term thiazolidinediones treatment can attenuate the effect of diabetes on rat atherosclerosis, OPG (osteoprotegerin) mRNA and expression increased.OPG may be involved in the occurrence of inhibition atherosclerosis lesion.

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