Dissertation
Dissertation > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Rheumatoid arthritis

Study on the Relationship between Serum High Mobility Group Box-1and Rheumatoid Arthritis

Author JiaZuo
Tutor TangHua
School Tianjin Medical University
Course Pathogen Biology
Keywords high mobility group box-1 high sensitivity C-reactive protein rheumatoid factor erythrocyte sedimentation rate rheumatoidarthritis active stage ameliorative stage
CLC R593.22
Type Master's thesis
Year 2009
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[Purpose]High mobility group box-1(HMGB1) was discovered as a member of a novel family of inflammatory cytokines in recent years. HMGBl is liberated to the extracellular milieu through two alternative mechanisms:it can be passively released from damaged or necrotic cells in injured tissues or actively secreted by immune cells including macrophages and neutrophils, it participates in the inflammatory process. Many evidences show that HMGB1expression is increased in the synovium of patients with RA, there are some arguments about the level of HMGB1in the serum, the study of serum HMGB1level in different disease stage of patients with RA were not find. This study is to determine the relationship between serum HMGB1level and disease status of patients with RA, and study the relationship between HMGB1level and other lab diagnostic parameters in patient with RA.[Methods]1.Using qualitative ELISA-kits to detect serum HMGB1level of patients with RA in different time:in-patient2days, after treatment2weeks,4weeks, control groups were detected only1time.2.Using erythrocyte sedimentation rate machine to detect erythrocyte sedimentation rate of patients with RA in different time:in-patient2days, after treatment2weeks,4weeks, control groups were detected only1time.3.Using automatic blood cell analysis machine to detect platelet and WBC results of patients with RA in-patient2days and compared with control group.4.Using automatic immune analysis machine to detect serum hs-CRP、RF、C3、 C4、IgG、IgM、IgA levels of patients with RA in-patient2days and continually supervised hs-CRP、RF after treatment2weeks,4weeks, control groups were detected only1time.【Results]1.In-patient2days results:serum HMGB1、hs-CRP、RF、 ESR results in active stage group higher than those of ameliorative stage group of patients with RA, the results of ameliorative stage group higher than those of control group, compare between three groups there has a significant difference, F=391.841, F=630.885, F=1872.404, F=385.549(P<0.01), there has a higher level of PLT and C3、C4、IgG、 IgM than those of control group (P<0.01), but there is no difference in two groups with RA (P>0.05).Comparing WBC and IgA results, there is no difference between RA and control group (P>0.05).2.The results has difference in two groups with RA were proceed continually supervise:The results of HMGB1、hs-CRP、RF、ESR in two groups with RA has a significant difference accompany the time change, F=934.248, F=664.439, F=1703.922, F=370.563, comparing between two groups has a significant difference, F=95.646, F=156.504, F=1720.155, F=176.412(P<0.01).3. Serum HMGB1level has a positive correlation with hs-CRP、RF、ESR results in patients with RA at different time, there is a significant difference (P<0.01). HMGB1with hs-CRP, r=0.684, r=0.585, r=0.321. HMGB1with RF, r=0.852, r=0.617, r=0.650. HMGB1with ESR, r=0.751, r=0.524, r=0.452.[conclusion]To predict serum HMGB1level of patients with RA and study the relationship between HMGB1level and the stage of RA, and study the relationship between HMGB1level and others check results in RA. The results show serum HMGB1level of patients with RA higher than those of control group, active stage patients higher than those of ameliorative stage, serum HMGB1is increasing accompany disease development, after treatment HMGB1is decreasing step by step, Serum HMGB1level has a positive correlation with hs-CRP、RF、ESR results, HMGB1plays an important role in incurring and development of disease.

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