Dissertation > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors

Application of DNA Quantitative Cytology of Cervical Intraepithelial in Early Screening Cervical Cancer

Author LiuXiaoZuo
Tutor WangJingBo
School Hebei North University
Course Human Anatomy and Embryology
Keywords Cervical cancer Cell DNA times body quantitative analysisDNA HPV16 and18-DNA subtype PCR Feulgen dyeing
CLC R737.33
Type Master's thesis
Year 2013
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The study mainly focused on evaluaing the sensitivity, specificity, positive predictive value, negative predictive value of DNA quantitative technique and HPV16,18-DNA subtype detection technology in application of diagnosising of cervical cancer, the standard of which was analysis of cell biopsy. With the comparative results, the clinical application value, the local incidence trend and age distribution were evaluated so as to provide scientific basis for the prevention and treatment of cervical cancer.The sample was collected from251hospital of the Chinese People’s Liberation Army and cooperated hospital during January2011and December2012. This cervical cell included2450patients whose age rage was20-65. The patients had more than two years of sexual history, but they had never taken any contraceptives or any other steroidal drugs. The cervical exfoliated cell of the above patients were Feulgen staining by Thin-Prep cytology test. Then, the Feulgen slices were scanned and DNA analysed by AcCell Automatic cell image analysis system, which were also tested by real-time fluorescent (FQ-PCR) on HPV16,18-DNA of the cast-off cells. Those patients whose DNA quantitative analysis test results were the cells of DNA aneuploid or HPV16.18infection were advised to have tissue biopsy on cervical microarray of3,6,9,12and were calculted the sensitivity, specificity, positive predictive value, and negative predictive value reopectively.DNA heteroploid cell combined with the postive pathological diagnosis were95.6%(151/158) inflammation,88.2%(30/34) CIN Ⅰ (Cervical intraepithelial neoplasia Ⅰ),95.5%(21/22) CINⅡ (Cervical intraepithelial neoplasiaⅡ),100%(11/11) CINⅢ (Cervical intraepithelial neoplasiaⅢ),100%(5/5) cervical cancer, total94.8%(218/230). HPV16,18-DNA infection combined with the postive pathological diagnosis were36.7%(58/158),44.1%(15/34) CIN Ⅰ,72.7%(16/22CIN Ⅱ),77.3%(8/11) CINⅢ,80.0%(4/5) cervical cancer. The age distribution was20~3029.7%(11/37),31~4049.0%(48/98),41~5046.9%(30/64),51~6043.5%(10/23),>6025.0%(2/8). This result showed that the relative high-incidence infection period was30~50. The DNA ploidy analysis sensitivity was78.94%and relative specificity was92.6%; the HPV16,18-DNA sensitivity was73.68%and relative specificity was97.1%. So the DNA ploidy analysis was more sensitive than the HPV16,18-DNA test, and they both were sensitive on CIN Ⅱ、CINⅢ precancerous lesions and the above.DNA content could reflect the degree of malignancy and biological behavior. Quantitative analysis of cellular DNA could predict cervical cancer and precancerous lesions more accurately, so it is useful on clinical diagnosis and prognosis evaluation by predicting the development of cervical disease. Cervical cancer caused by HPV infection was the only human cancer that had a definite cause. HPV DNA could be detected more than90%in cervical cancer sample, and the most common one is HPV16/18subtype. Both methods have a high sensitivity on cervical cancer and precancerous lesions; but nuclear DNA ploidy was detected by DNA ploid analysis, which was objective and accurate. This mothed could obtain the tumor biological characteristics which was difficult to obtain the information from morphology and it also could screen lesions sample with malignant change trend before the lesions occurred in cervical cells. So DNA ploidy analysis of HPV16,18-DNA subtypes test was more effective on solving the problem of lack of large-scale cervical cancer screening and experience-based cytology doctors. Meanwhile, the method was more sensitive and reasonable to women’s cervical cancer screening.

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