Primary Study on the Inhibition of Formation of Intracranial Aneurysm by an Inhibitor of SRK Kinase-PP2
|Keywords||Intracranial aneurysm Sprague-Dawley Animal model PP2 PP3 Elastin Van Gieson Stain HE Staining|
Objective：The etiology of the intracranial aneurysm is unclear. In our previous study it wasfound that SRC kinase may play a certain role to some extent in the formation of theintracranial aneurysm by regulating the phosphorylation of vascular endothelialcadherin (VE-cadherin）and its relevant signal pathway. This paper is designed tostudy the inhibition of the formation of intracranial aneurysm by PP2, an inhibitor ofSRC kinase.Methods：160male SD rats were divided into two groups randomly, of which140ratswere used to create experimental intracranial aneurysm animal model, and theremaining20rats as the nomal control.140male SD rats were subdivided into7sub-groups,which included40-μg and20-μg PP2treatment sub-groups at early stage,40-μg and20-μg PP2treatment sub-groups at late stage,40-μg and20-μg PP3treatment sub-groups at early stage,40-μg and20-μg PP3treatment sub-groups at latestage and the positive control group.The vessels at the bifurcation of right anteriorcerebral artery-olfactory artery (ACA-OA) were sampled to observe the formation ofthe intracranial aneurysms.Results：The formation rate of intracranial aneurysm was20%（4/20）in both sub-groupsof40-μg and20-μg PP2treatment at early stage,40%（8/20）and45%（9/20）insub-groups of40-μg and20-μg PP2treatment at late stage respectively,50%（10/20）and55%（11/20） in sub-groups of PP3treatment at early and late stagerespectively,the fomation rate of aneurysm in the positive control group was55%. Noaneurysm was found in the nomal control group.Compared with the positive control subgroup, the formation rate of intracranialaneurysm in both sub-groups of40-μg and20-μg PP2treatment at early stage wassignificantly decreased (p<0.05）, but there was no difference between othersubgroups （p>0.05）. Conclusion：PP2,an inhibitor of SRC kinase can obviously inhibit the formation of ratexperimental intracranial aneurysm when used at early stage after the induction ofexperiment aneurysm; however, the inhibition could not found when it was used atthe late stage after the induction of experiment aneurysm. Nevertheless,our resultsfirstly confirmed the inhibition of the formation of intracranial aneurysms in vivo bya SRC kinase inhibitor.