Dissertation > Medicine, health > Oncology > General issues > Tumor Therapy

Preliminary Study on Therapeutic Effect of Combination of Several Genes on the Growth of H22Tumor

Author ZhouZuo
Tutor ZhangGuiMei
School Huazhong University of Science and Technology
Course Biochemistry and Molecular Biology
Keywords Tumor therapy gene therapy combined treatment IFN-β TNF-α CXCL-1
CLC R730.5
Type Master's thesis
Year 2012
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Objective: To investigate the inhibitory effect of combination of severaldifferent cytokine genes on the H22tumor.Analyzing the best concentrate andcombination of the gene therapy on H22tumor.Methods:(1) Tumor model was established by inoculation of H22hepatocarcinoma cells at right hind thigh of BALB/c mice.(2) Tumor gene therapywas performed by in vivo gene transfection with the method of injection of nakedplasmid into tumor to express polypeptide IFN-βTNF-αCXCL-1、CH50.(3) Tumorgene therapy was performed by in vivo gene transfection with the method based onhydrodynamics to express polypeptide IFN-β.(4) Tumor weight was used to reflectthe the inhibitory effect of different polypeptides on tumor growth.(5)Combination of the best dose of different polypeptide to the tumor-bear mice, andevaluate the advantages of the combination therapy.Results:(1) The best dose of the pcDNA3.1-IFN-β plasmid is100μg/100μlwith the hydrodynamic tail vein delivery method.(2)The best dose of thepcDNA3.1-TNF-α plasmid is10μg/100μl with injection into into the tumormicroenvironment.(3)The best dose of the pcDNA3.1-CXCL-1plasmid is10μg/100μl with injection into into the tumor microenvironment.(4)Combining above threeplasmids, we can get stronger suppressive effect on tumor growth than any one ofthem.Conclusions: Combining a variety of antitumor cytokine’gene with differentways has a better antitumor effect than using one kind of cytokines at one time.

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