Dissertation
Dissertation > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia

Preliminary Study in the Significance of Protease Nexin-1and Metallothionein in Adult's Acute Myeloid Leukemia

Author HeJing
Tutor ZhouJianFeng
School Huazhong University of Science and Technology
Course Department of Hematology
Keywords acute myeloid leukemia protease nexin-1 metallothionein apoptosis
CLC R733.71
Type Master's thesis
Year 2013
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Objective1. To investigate the regulatory role of protease nexin-1to metallothionein inthe chronic myeloid leukemia K562cell line.2. To explore the expression and significance of protease nexin-1andmetallothionein in newly diagnosed acute myeloid leukemia’s bone marrow biopsiesof adults’.Methods1. We infused the PN-1plasmid into K562cell line by electric transfection anddetected expression change of MT mRNA and PN-1mRNA with real-time PCR,which could verify the regulatory role of protease nexin-1to metallothionein in ourprevious gene chip.2.29bone marrow biopsies of newly diagnosed acute myeloid leukemia’s and8of iron deficiency-anemia were detected by immunohistochemistry to investigate theexpression level of protease nexin-1and metallothionein.Results1. With the expression level of PN-1mRNA elevated, the expression level ofMT1B mRNA,MT1E mRNA,MT1G mRNA,MT1H mRNA were reduced in K562cell line after PN-1plasmid transfection, which was consistent with our previousgene chip.2. The positive credits of MT in bone marrow biopsies of AML and withoutblood malignancies were1.88±1.79、0.76±0.84, respectively. The result wasstatistically significant (P=0.038). 3. The positive credits of PN-1in bone marrow biopsies of AML and withoutblood malignancies were0.56±0.82、0.54±0.47, respectively. The result had nostatistical significancy (P=0.305).Conclusion1. Protease nexin-1had negative control on metallothionein in CML K562cellline.2. IHC credits of metallothionein in AML bone marrow biopsies weresignificantly higher than in bone marrow biopsies without blood manignancies. Itmeant that MT may involve in the malignant proliferation, apoptosis inhibition andother sectors of leukemia cells.

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