Dissertation
Dissertation > Medicine, health > Oncology > Respiratory system tumors > Lung tumors

Chances of ISR in A549Cells before and after Radiotherapy and Cnemotherapy and Its Significance

Author LiZhen
Tutor ZhangBenHua
School Taishan Medical College
Course Oncology
Keywords lung cancer A549cells Insulin receptor Immunohistochemistry Radiosensitization Docetaxel Targeted therapy Delivery time
CLC R734.2
Type Master's thesis
Year 2013
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purposeDo research on some changes of insulin receptor (Insulin, Receptor, ISR) oflung cancer A549cells before and after radiotherapy and chemotherapy. Inaddition to this, we have to do some studies on these changes which areassociated with chemotherapy and radiotherapy to find some feasible biologicalindicators as sensitive indexes to evaluate the efficacy of radiotherapy andchemotherapy. Finally, by some indexes, we can realize the individualizedtreatment of lung cancer.methodsPut lung adenocarcinoma cells A549which are in logarithmic phase ofgrowth and grow in good addition singled out, randomized, divided into thecontrol group (not given high energy X-ray radiation and chemotherapy drugdocetaxel) and the experiment group (given certain concentration of docetaxeland high energy6MVX-ray radiation, including2GY,4GY,6GY,8GY,10GY,12GY in different time). After radiotherapy and chemotherapy, the cells of thecontrol group and the experimental group have been culturing for7days. Duringthe period, change culture medium on time. After that, definite quantities ofinsulin receptor (Insulin, Receptor, ISR) of lung cancer A549cells by themethod of immunohistochemistry and analyze changes after radiotherapy andchanges before and after radiotherapy and chemotherapy in different sequencesand the relationship between changes and radiotherapy and chemotherapy. Results(1) The insulin receptor (ISR) is difussed on cell membrane and incytoplasm of some cells. It appears in the form of small particles.The result ison the basis of the depth of the color of small particles on cell membrane. Thelight color is defined as+(positive) and the deep color is defined as+++(strong positive). In addition, the result recognize the positive expression ratesand the strong positive expression rates of the insulin receptor as quantitativebasis.(2) At the same dose (6GY radiation), the positive expression rates and thestrong positive expression rates of the insulin receptor is: the group of simpleirradiation>the group of irradiation and docetaxel after irradiation> the groupof irradiation and docetaxel after irradiation immediately> the group ofirradiation and docetaxel before irradiation. At the Small dose (2GY and4GY),there are no differences among these groups on the strong positive expressionrates of the insulin receptor. So it is no statistically significant.(P>0.05).(3) In the group of given radiotherapy only, as the dose increases, thepositive expression rate of insulin receptor decreases. From6GY to12GY, as thedose increases, the strong positive expression rate of the insulin receptorgradually increases. But they are lower than the group of given small doses(2GY and4GY).(4) In the group of irradiation and docetaxel before irradiation, as the doseincreases, the positive expression rate of insulin receptor decreases. From6GYto12GY, as the dose increases, the strong positive expression rate of the insulinreceptor gradually increases. But they are lower than the group of given smalldoses (2GY and4GY).(5) In the group of irradiation and docetaxel after irradiation immediately,as the dose increases, the positive expression rate of insulin receptor decreases.From6GY to12GY, as the dose increases, the strong positive expression rate ofthe insulin receptor gradually increases. But they are lower than the group ofgiven small doses (2GY and4GY).(6) In the group of irradiation and docetaxel after irradiation, as the doseincreases, the positive expression rate of insulin receptor decreases. From6GYto12GY, as the dose increases, the strong positive expression rate of the insulin receptor gradually increases. But they are lower than the group of given smalldoses (2GY and4GY).Conclusions(1) The insulin receptor exists in cell membrane of lung adenocarcinomaA549cells, providing a carrier for insulin PI3K/Akt channel and Ras/ERKchannel, so as to promote proliferation and apoptosis of cells.(2) After incoluated3~5days,normal lung adenocarcinoma A549cellsto normal after3~5days after a logarithmic growth phase, for the platform, thecell growth rate. Given radiotherapy and chemotherapy, the growth rate of thecells decreased significantly, many cells into apoptosis.(3) For the simple irradiation group, positive control group and small dosegroup the expression of insulin receptor and strong without obvious difference,high dose (6GY as) positive expression of insulin receptor strong rate higherthan that of the control group, there was statistical significance, that the ray ismainly on the expression of insulin receptor positive cells were killing, receptorexpression the positive cells showed resistance to radiation.(4) The same dose drug group, positive expression of insulin receptorstrong rate is higher than the simple irradiation group, chemotherapy drugdocetaxel shows with radiosensitivity of lung adenocarcinoma A549cells.(5)The same dose, cell insulin receptor positive rate and the strong positiveexpression rate: after irradiation+docetaxel group> immediately afterirradiation plus docetaxel group> before irradiation plus docetaxel group, thatdocetaxel combined with radiotherapy to radiotherapy was administered beforeShe Zengmin effect on lung adenocarcinoma cell A549best.(6) Chemotherapy drug positive cells and positive cells were killing ofinsulin receptor, strong positive expression cells stronger damage on insulinreceptor.(7)In the guidance of clinical treatment, some drug targeting applicationsthat provide reference basis, can consider the application of some of theinhibitors, specific blocking or interfere with insulin in the signal transductionof cells, providing new ideas for the treatment of lung cancer.

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