Dissertation
Dissertation > Medicine, health > Oncology > Respiratory system tumors > Lung tumors

Effects of ILK on Production of Tumor Immune Escape Related Factors of A549Cells Induced by LPS

Author Liu
Tutor ChenQuan
School Chongqing Medical University
Course Immunology
Keywords Integrin linked kinase (ILK) Lipopolysaccharide (LPS) Lung cancer Immune escape Cytokine
CLC R734.2
Type Master's thesis
Year 2012
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Objective:Based on a lung cancer A549cell line stable over-expressing human Integrin-linked kinase (ILK), to explore the effects of ILK on that lung cancer A549cells produce VEGF, TGF-P and IL-6, immune escape related cytokines, induced by Lipopolysaccharide (LPS).Methods:The A549cells line with over-expressing ILK as experimental group was stimulated by LPS, meanwhile, the blank A549cells as control and the saline as negative control factor, then the VEGF, TGF-β and IL-6, immune escape related cytokine, in cell culture lysates and supernatants were determined quantitatively by ELISA respectively and a statistical analysis of the test results were performed too.Results:The independent samples t-test analysis were performed to the quantitative test results VEGF, TGF-P and IL-6. The cell lysates compared with culture supernatants, the three factors had no significant difference (P>0.05); The A549cells line with over-expressing ILK compared with blank A549cells, the three factors were significantly higher (P<0.05); The blank A549cells stimulated by LPS compared with ones stimulated by NS control, there were significant differences in the expression of VEGF, IL-6and TGF-β (P<0.05); The A549cells line with over-expressing ILK stimulated by LPS compared with ones stimulated by LPS, there were significant differences in the expression of VEGF, IL-6and TGF-β; The A549cells line with over-expressing ILK stimulated by LPS compared with blank A549cells stimulated by LPS, the expression of VEGF, TGF-β and IL-6were significantly increased (P<0.05).Conclusion:LPS or ILK over-expression could promote A549cells to produce VEGF, TGF-β and IL-6and the role was more obvious when two factors existed on the cells at the same time. The experimental results show that ILK may play a regulating role in the signaling pathways of promoting tumor effect of pro-inflammatory cytokines.The study laid the foundation to further explore the relationship between ILK, inflammation and tumor, also further confirmed the feasibility that the ILK over-expression A549cell model is applied to the study on the effect and mechanism of ILK.

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