Scorpions amplification refractory mutation gene mutation of epidermal growth factor receptor system detecting serum and tumor tissue
|School||Beijing Union Medical College|
|Keywords||Lung cancer Epidermal growth factor receptor Polymerase chain reaction Serum|
ObjectiveTo detect the EGFR mutations in tumor and matched serum in lung cancer patients by scorpions amplification refractory mutation system (Scorpions ARMS).MethodsSpin column method was used to extract DNA from formalin fixed paraffin samples and three methods (spin column, magnetic beads, and phenol chloroform) were used to extract cell-free DNA from serum.35tumor samples and35matched serum samples from patients with lung cancer were analyzed for EGFR mutations in exon18,19,20and21by Scorpions ARMS.ResultsEGFR mutations were detected in the tumor samples of19patients (54.3%) and in the serum samples of6patients (17.1%). EGFR mutation status in the tumors and serum samples was consistent in21(60%) of the35pairs. EGFR mutation rate in the serum of stage Ⅲ and Ⅳ patients (35.7%,5/14) was significantly higher than in stage I and Ⅱpatients (4.5%,1/21)ConclusionsCell-free DNA in serum offers the possibility of monitoring EGFR mutations in primary tumors in stage Ⅲ and Ⅳ patients with lung cancer. Scorpions ARMS is valid and effective for the detection of EGFR mutations. Magnetic beads method is effective for extracting DNA from serum.