Fluorouracil Sustained-release Preparation on Colorectal Cancer in Nude Mice Subcutaneous Implanted Tumor Growth and Bcl-2/Bax Protein、Survivin/Caspase-3Expression Effect
|School||Guilin Medical College,|
|Keywords||Fluorouracil slow-release formulation Colorectal cancer nude miceplanting tumor Bcl-2/Bax Survivin/Caspase-3|
Objective:Observe the slow release agents for people fluorouracil colorectal cancer nude mice subcutaneous tumor growth and Bel-2/Bax protein, Survivin/Caspase-3influence. Explore fluorouracil slow-release agents for people nude mice subcutaneous tumor colorectal cancer grow inhibition, Preliminary clarify fluorouracil slow-release formulation of induce the apoptosis of mechanism, Fluorouracil slow-release formulation for the clinical application of antitumor to provide the basis.Methods:Build BALB/c nude mice subcutaneous planting tumor model, randomly divided into three groups:the injected respectively vein control, the tail vein into PBS5-FU injection group, the tumor body injected directly into the slow-release agent groups, and only8.1.Record three groups nude mice tumor size, tumor growth of planting more each group, draw tumor growth curve, tumor inhibition rate calculation.2.HE dyeing observation group histopathologic change tumor.3.1mmunohistochemical method to detect Bcl-2/Bax protein expression level of change.4.Half quantitative RT-PCR method to detect the nude mice the tumor body Survivin/Caspase-3in the level of expression.Results:1.Nude mice subcutaneous tumor cells after inoculation7d.Randomly take2-3will only nude mice executed, and take transplant tumors of HE dyed. HE dyed see organization cells are arranged into irregular glands shape, have nuclear division phase, success tumorigenic and into tumor rate was90%. After drawing tumor growth curve and30days tumor size:PBS for control group (3240+187) mm3,5-FU injection chemotherapy group for (1568+86) mm3, slow-release agent implant group (600±38) mm3, differences are statistically significant (P<0.05).2.HE dyed PBS tumor tissue pathology control group characteristics:400times in a microscope to, the gland tumor cells form, the tumor cells have a few apoptosis, a flake necrosis.100times in a microscope to, tumor tissue to fat, muscle invasion of organization. HE dyed5-FU histopathological features of injection cancer:400times in a microscope to, tumor cells heterosexual type obvious, degree of malignancy, stronger, gland tubular structure is not obvious, the plot of the neoplastic cells have large apoptosis, a little necrosis. In100times to a microscope, a local tumor tissue to invade adipose tissue. HE dyed slow-release agent implanted histopathological features of cancer:400times in a microscope to, tumor cells heterosexual type obvious, malignant significantly, gland tubular structure is not obvious, the tumor cell apoptosis is obvious, only a very small necrosis.100times in a microscope a tumor, clear organization boundary, did not see the fat and muscle layer by organization, slow growth.3. Immunohistochemical method to detect Bcl-2/Bax protein level express found:5-FU injection chemotherapy group, slow-release agent implant group Bcl-2protein expression are lower than those of the control group PBS, Bax expression are higher than the PBS control;5-FU injection chemotherapy group Bcl-2below slow-release group, Bax higher than slow-release group.4. Half quantitative RT-PCR method to detect the nude mice the tumor body Survivin/Caspase-3in expression shows:Survivin:Slow-release agent implant group less than5-FU injection group,5-FU injection group less than PBS control group, Caspase-3:slow-release agent implant group above5-FU injection group,5-FU injection group higher than PBS control group.Conclusion:1. Fluorouracil slow-release preparations can obviously restrain the growth of tumors nude mice subcutaneous planting.2. Fluorouracil slow-release formulation can restrain Survivin expression and raised Caspase-3expression, this may be fluorouracil slow-release formulation mediated tumor cell apoptosis realize anticancer mechanisms of one.3. Fluorouracil slow-release formulation can cut Bcl-2, raise the expression of Bax, this may be fluorouracil slow-release formulation mediated tumor cell apoptosis realize anticancer mechanisms of one.