Effects of Glutamine on Intestine Mucosa Morpholopy and Synthesis of Cellylar Energy in Weaned Piglets
|Course||Animal Nutrition and Feed Science|
|Keywords||Glutamine Weaned piglets Intestinal villi Light microscopy sections Electron microscopy sliced ATP|
In this experiment, 21-day-old piglets as the object studied glutamine on intestinal villus morphology and weaned piglets cellular energy synthesis. Optional 90 Duroc piglets were randomly divided into 2 groups: control group and 1% glutamine group, each treatment three replicates of 15 piglets. Control group was fed basal diet, the test group add another 1% glutamine. In the beginning of the test and the first 7 days after weaning, 14 days, 21 days of piglets weighed individually arrive at the average daily gain (ADG), average daily feed intake (ADFI), feed to gain ratio (F / G). Recorded daily diarrhea, diarrhea rates obtained. In the day of weaning, weaning 7d, after weaning 14d, per kilogram of body weight oral perfusion 10% D-xylose solution lmL, serum, -70 ℃ preservation, phloroglucinol colorimetric determination of serum D-xylose concentration. Piglets weaned at 21 days of age, respectively, in the day of weaning, weaning after 3 days, 5 days, 7 days, 14 days, slaughter pigs sampled. Slaughter serum, serum biochemical parameters were measured and total protein, blood urea nitrogen (BUN), glutamine, disaccharide enzyme (total). Take bowel biopsy samples produced by light microscopy and light microscopy observation of intestinal villi biopsy morphology. Mucosal samples measured main aspects of cellular energy measured ATP, cyclic guanosine monophosphate (cGMP), cyclic adenosine monophosphate (cAMP), nitric oxide (NO), nitric oxide synthase (NOS) and other indicators. Feeding trial results showed that 1% Gln diets can significantly improve weaning average daily gain, feed conversion ratio and diarrhea rates. 21-28 days average daily gain of 1% Gln group than the control group increased 27.99% (P lt; 0.05); 28-35 age group than in the control group test increased 16.85% (P lt; 0.05); 35 - 42-day experimental group than in the control group increased 20.49% (P lt; 0.05). The test period feed conversion ratio test group than the control group, the difference was significant (P lt; 0.05). Dietary Gln can significantly reduce diarrhea in weaned piglets rate (P lt; 0.05) xylose test results show that, Gln can promote intestinal absorption function after weaning serum D-xylose concentration decreased significantly, adding Gln can improve intestinal tract absorption. Weaning 7 days, 14 days, 21 days xylose concentration test group were higher than the control group 62.01% (P lt; 0.05), 33.03% (P lt; 0.05), 42.71% (P lt; 0.05). Serum biochemical test showed that weaning stress significantly decreased serum total protein levels. But weaning stress test did not reduce serum total protein, weaning three days, five days, seven days test serum total protein content were higher than the control group, 41.42% (P lt; 0.01), 16.53% (P lt; 0.05 ), 22.11% (P lt; 0.05). Glutamine to a certain extent, reduce the level of serum urea nitrogen, weaned 3 days, 5 days, 7 days, 14 days trial group urea nitrogen levels were lower than the control group 39.48% (P lt; 0.01), 12.31%, 29.34% (P lt; - .05), 30.64% (P lt; - .05). After weaning diets supplemented with 1% Gln prevents atrophy of the villi and crypts deepened. 5 days after weaning, each group duodenum, jejunum and ileum villus atrophy to varying degrees, but the 1% Gln group villus height, respectively, than the control group 18.38% (P lt; - .05), 9.94%, 27.44 % (P lt; - .01). 7 days after weaning, Gln group villus height respectively, compared with the control group 22.42% (P lt; - .05), 18.17% (P lt; - .05), 13.63% (P lt; - .05). After weaning, each group small intestine villi width has increased in varying degrees, weaning five days villus width Gln group than the control group, respectively, 24.45 (P lt; - .01), 37.45 (P lt; - .01). 21.10% (P lt; - .05). Piglets after weaning crypts have different levels of deepened. Weaning 5 days, the control group, duodenum, jejunum, ileum crypt depth ratio of the test group were deep 18.80% (P lt; - .05), 14.48%, 23.94% (P lt; 0.05). SEM results showed that dietary supplementation Gln can significantly promote the development of intestinal villi, to prevent loss and damage fluff. Dietary Gln significantly increased duodenum, jejunum, ileum mucosa ATP content. Weaning three days test group duodenum, jejunum, ileum mucosal ATP content were higher than control group 19.30% (P lt; 0.05), 24.94% (P lt; 0.05), 17.79% (P lt; 0.05); weaning 5 days were higher by 18.19 (P lt; 0.05), 32.74% (P lt; 0.05), 27.28% (P lt; 0.05). Dietary Gln can significantly increase weaning three days duodenum, jejunum NO content, the experimental group were higher than the control group, 47.5% (P lt; 0.05), 43.38% (P lt; 0.05). Bowel mucosa of the small intestine after weaning NOS decreased, but the difference was not statistically significant. Intestinal mucosal cAMP content in each group at weaning three days have significant differences, other phases were no significant differences. Weaning three days, the test group duodenum, ileum mucosa cAMP levels were higher than control group 32.75% (P lt; 0.05), 34.05% (P lt; 0.05). To sum up: Dietary glutamine can improve growth performance, reduce intestinal mucosal atrophy, intestinal mucosa to promote the synthesis of ATP content.