The Effects of Silage Additives on the Ensiling Fermentation of Green Corn Stalk Silage`
|School||Chinese Academy of Agricultural Sciences|
|Course||Animal Nutrition and Feed Science|
|Keywords||Silage additives And without spike green corn stalks Fermentation Quality Aerobic stability Nitrate|
In this study, using laboratory silage microbiological techniques, analysis of chemical composition and in vitro digestion research tools, research Lactobacillus plantarum, molasses, formic acid, Lactobacillus buchneri and nitrates on without spike green corn stalks silage fermentation process affect the quality and fermentation. Mainly include the following: test molasses, Lactobacillus plantarum and formic acid with a spike of green corn stalks silage fermentation process and quality without spike green corn stalks for silage raw materials, set the following four treatment groups: control group, molasses treatment group (was 4%), Lactobacillus plantarum treatment group (dosage 106 cfu / g FM) and formic acid treatment group (dosage 0.4%). In the fermentation 1,3,7,15,30 and 60 days, respectively indicators cans sampling, test samples of lactic acid bacteria and fungi content and pH, lactate, VFA, NH3-N and dry matter digestibility. The results show that: Add the molasses ensiling process pH did not significantly significant impact (P gt; 0.05), there is increase in lactic acid bacteria and fungi content of trends, reduced silage process acetic acid content (P lt; 0.05), to improve the fermentation process lactic acid acetic acid ratio the fermentation stage silage dry matter content and residual water-soluble carbohydrate content (P lt; 0.05), reduced silage dry matter digestibility (P lt; 0.05); adding Lactobacillus plantarum increase spiked green corn stalks trend of lactic acid bacteria content of the silage fermentation process, increase the pH of the fermentation end (P LT; 0.05), increase in lactic acid content of the fermentation process trend (P GT; 0.05), and improved acid acetic acid ratio, improve the final fermented silage dry matter content (P lt; 0.05), to reduce the residual water-soluble carbohydrate content, fermentation final crude protein and dry matter rate (P lt; 0.05); adding formic acid to reduce without spike green corn stalks silage fermentation process lactic acid bacteria and fungi trend of the content of the trend of the pH to increase the fermentation process reduces the lactic acid and acetic acid content of the fermentation process (P LT; 0.05), increased fermentation final residual water soluble carbohydrate content (P LT; 0.05) to reduce the fermentation final dry matter content and dry matter digestibility (P lt; 0.05). Test two Lactobacillus buchneri spiked green corn stalks silage fermentation quality and aerobic stability of silage raw materials without spike green corn stalks, respectively 0,3.05 × 104,3.05 × 105,3.05 × 106 cfu Add / g FM Lactobacillus buchneri for silage. Fermentation 1,8,16,21,26,34 and 60 days respectively cans sampling, testing samples in lactic acid bacteria and fungi content and pH, lactate, VFA, NH3-N, dry matter digestibility and aerobic stability indicators. The results show that: add Lactobacillus buchneri reduced without spike green corn stalks the silage process of fungal content trend, the trend of increasing pH and acid content, reduce the proportion of the content of lactic acid and lactic acid acetic acid, add Lactobacillus buchneri reduce fermentation final silage dry matter content, dry matter recovery and dry matter digestibility (P lt; 0.05), to improve the aerobic stability of silage, extended open cans save time, the Lactobacillus buchneri add the amount of the greater The aerobic stability increasing trend. Test three different moisture conditions, potassium nitrate role without spike green corn stalks silage fermentation process without spike green corn stalks for silage raw materials, 40%, 33% and 25% three dry matter level, each dry matter levels were added the potassium nitrate 0 and 1.5 and 3.0g/kg FM. In fermentation 0,3,7,15,30 and 60 days, respectively, to open the tank sampling, the detection pH, lactate, VFA, NH3-N, NO3-N and NO2-N content and other indicators. The results showed that: no apparent regularity under different water conditions, potassium nitrate silage pH, lactic acid, acetic acid and NH3-N content. Add to silage raw potassium nitrate, silage nitrate and nitrite content increased. Silage nitrate degradation occurred early in the silage. After fermentation, the silage nitrate degradation rate between 66.76% -92.69%, nitrate and nitrite content in the following 900mg/kgDM and 1.35mg/kgDM, not enough to endanger animal health.