Dissertation > Medicine, health > Oncology > General issues > Tumor Therapy

The Study of the Therapic Effect of Exercise Combining with Paclitaxel-loaded Nanoparticles Drug Delivery Systems in Cancer

Author MaZhiChao
Tutor ZhangJun;DuanYouRong
School Yangzhou University
Course Human Movement Science
Keywords Movement Tumor Nanoparticles Polylactic acid / polyethylene glycol Taxol
CLC R730.5
Type Master's thesis
Year 2010
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As a useful adjuvant treatment of tumors in the campaign to maintain or improve the survival of cancer patients quality role gradually been accepted. More and more on the research work of the nanoparticle drug delivery system is being widely carried out. Movement and nanoparticle drug delivery system in the prevention, adjuvant therapy, and treatment of tumors, have played a positive role. However, the movement joint nanoparticle drug delivery system reported treatment for solid tumors is still rare. Malignancy is a serious disease threat to human health and life, for the prevention and treatment of tumors is an important topic of common concern for the medical profession and academia. With the in-depth understanding of tumor pathogenesis and biological characteristics of the continuous improvement of early diagnosis of tumor and adjuvant therapy, systemic treatment of tumor increasingly important. Rehabilitation exercise as an adjunct therapy combined with conventional treatment (surgery, radiotherapy, chemotherapy and traditional Chinese medicine treatment) is gradually being applied in cancer therapy, the common concern of the majority of clinicians and researchers. In recent years, domestic and foreign scholars have conducted a lot of research and found that the movement caused by surgery, radiation therapy, chemotherapy, cancer patients can improve heart and lung function and physical function lower state to improve cancer fatigue caused due to tumor alleviate heavy psychological burden on patients, improve the patient's quality of life. To explore the role of movement in the adjuvant treatment of cancer and its possible mechanism of action of this article and summarized clinical and scientific literature at home and abroad in recent years about the impact of the movement of the tumor, analysis of the movement of the role of adjuvant therapy for breast cancer , and on this basis for the movement as an adjunctive treatment in cancer therapy, there may be the mechanism to make a shallow analysis. This study on the basis of previous studies, the drug combination sport of Lewis lung C57BL / 6 mouse animal model experiments to study the drug combined with the impact of the movement of the tumor, and explore the the movement auxiliary tumor medication part mechanism for the movement in the auxiliary provide a theoretical basis for the treatment of tumors. Experimental method: Experiment 1, the use of high performance liquid chromatography evaluation of nanoparticle drug encapsulation efficiency and drug loading. Using laser light scattering spectrophotometer and transmission electron microscopy analysis of the particle size and structure of the nanoparticles. 37 ℃, pH value of 7.4 phosphate buffer to simulate the process of the release of the drug. And to verify the therapeutic effect of the drug-loaded nanoparticles prepared by a different preparation method on Lung load mice,, nanoparticles prepared filter out the effect of the most ideal method. Experiment 2, using laser confocal microscopy nanoparticles gathered in the Lewis lung carcinoma cells under in vitro conditions. Load of Lewis lung carcinoma cells of female C57BL / 6 mice were randomly divided into six groups: (1) saline group; (2) paclitaxel group; (3) the exercise group; (4) contains paclitaxel nanoparticles group; (5) paclitaxel plus exercise group; (6) a the paclitaxel nanoparticles joint exercise group. The drugs injected mice were intraperitoneally, and give related mice moderate-intensity exercise load for 12 days. Tumor volume was measured daily. The last day, the use of flow cytometry analysis of the phagocytic ability of mouse peritoneal macrophages, and an acetonitrile extract of the blood and tissue of mice were analyzed using high-performance liquid chromatograph. Experimental results: direct dissolution method and the dialysis nanoparticles prepared in case of a similar drug loading and encapsulation efficiency. Higher due to the the stereocomplexation effect, only by L-polylactic acid / polyethylene glycol or dextrose polylactic / polyethylene glycol compared to the nanoparticles constituted by the two copolymers constituting the hybrid nanoparticles display. drug encapsulation efficiency and drug loading. And compared to the dialysis method, direct dissolution of the prepared nanoparticles showed a faster release rate. Compared with the formulations used in clinical and dialysis prepared nanoparticles perfection spring nanoparticles prepared by direct dissolution carrier showed the strongest anti-cancer effect. Vivo test data show that: nanoparticle delivery system to improve the paclitaxel bioavailability in mice; movement of the phagocytic activity of macrophages influence; motion as an adjunct to improve the therapeutic effect of the carrier paclitaxel nanoparticles . Conclusion: The prepared by direct dissolution of L-and D-polylactic acid / polyethylene glycol mixed nanoparticles exhibit a number of advantages, such as ease of preparation, and does not use toxic organic solvents, as a carrier of hydrophobic drugs shows huge potential. Nanoparticle drug delivery system to improve the taxol in vivo bioavailability. On the other hand, the movement as an auxiliary means of intervention, and enhance the therapeutic effect of the paclitaxel-carrying nanoparticles.

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