The Identification and Classification of Olfactory Ensheathing Cells in Mouse
|School||Northeast Normal University|
|Keywords||Spinal cord injury OECs Primary cultured GFAP|
Spinal cord injury , even partial damage can also cause serious consequences , may cause the local permanent paralysis , seriously affecting the quality of life of patients , severe burden and loss will be brought to the patient 's family and society . The researchers found that spinal cord injury by cell transplantation , gene therapy or transgenic cell transplantation to repair . Studies have shown that in many transplant to repair the cells of the central nervous system damage candidate , OECs are classified as the most ideal of a cell . OECs on the cell membrane and the cell adhesion and promote nerve regeneration - related molecules , these molecules can create a good internal environment for the regeneration of axons help damaged axon elongation growth, promote axon extends to the target to help the recovery of neurological function . At present, the data show that OECs can promote sciatic nerve regeneration in mice can promote hemisection model mice tract axons extending growth . Now , more mature olfactory ensheathing cells in vitro method is the NASH poor attachment method . NASH differential adherence method using fibroblast cells , astrocytes and ensheathing cells adherent time to separate them . The cultured OECs can exhibit a variety of cell morphology and cell surface markers . The most common , there are two main , a neural membrane cell type to express the p75NTR receptor . Mainly distributed in the outer periphery of the olfactory neural system , was spindle . The species is a class of star-shaped glial cell type is mainly distributed in the mouse olfactory bulb , the majority of flat . Lack of p75NTR receptor , but GFAP into immunoreactive . We GFAP immunohistochemistry identification purification of olfactory ensheathing cells cultured in vitro . In this study, using a modified method of NASH differential adherence and cytarabine purified and cultured mouse olfactory bulb, the primary olfactory ensheathing cells using GFAP identified in vitro cell purity . Cell sorting using anti- PSA-NCAM and to anti- the two antibodies L-NGFr will culture , try of OECs different phenotype relationship , and hopes to further the different functions of the different strains of OECs was found on the cell expressed and substance secretion , and ultimately used in transplant the repair of spinal cord injury and promote axon regeneration .