Dissertation > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord

The Identification and Classification of Olfactory Ensheathing Cells in Mouse

Author QiaoXiaoFei
Tutor SunZuo
School Northeast Normal University
Course Genetics
Keywords Spinal cord injury OECs Primary cultured GFAP
CLC R651.2
Type Master's thesis
Year 2010
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Spinal cord injury , even partial damage can also cause serious consequences , may cause the local permanent paralysis , seriously affecting the quality of life of patients , severe burden and loss will be brought to the patient 's family and society . The researchers found that spinal cord injury by cell transplantation , gene therapy or transgenic cell transplantation to repair . Studies have shown that in many transplant to repair the cells of the central nervous system damage candidate , OECs are classified as the most ideal of a cell . OECs on the cell membrane and the cell adhesion and promote nerve regeneration - related molecules , these molecules can create a good internal environment for the regeneration of axons help damaged axon elongation growth, promote axon extends to the target to help the recovery of neurological function . At present, the data show that OECs can promote sciatic nerve regeneration in mice can promote hemisection model mice tract axons extending growth . Now , more mature olfactory ensheathing cells in vitro method is the NASH poor attachment method . NASH differential adherence method using fibroblast cells , astrocytes and ensheathing cells adherent time to separate them . The cultured OECs can exhibit a variety of cell morphology and cell surface markers . The most common , there are two main , a neural membrane cell type to express the p75NTR receptor . Mainly distributed in the outer periphery of the olfactory neural system , was spindle . The species is a class of star-shaped glial cell type is mainly distributed in the mouse olfactory bulb , the majority of flat . Lack of p75NTR receptor , but GFAP into immunoreactive . We GFAP immunohistochemistry identification purification of olfactory ensheathing cells cultured in vitro . In this study, using a modified method of NASH differential adherence and cytarabine purified and cultured mouse olfactory bulb, the primary olfactory ensheathing cells using GFAP identified in vitro cell purity . Cell sorting using anti- PSA-NCAM and to anti- the two antibodies L-NGFr will culture , try of OECs different phenotype relationship , and hopes to further the different functions of the different strains of OECs was found on the cell expressed and substance secretion , and ultimately used in transplant the repair of spinal cord injury and promote axon regeneration .

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