Dissertation
Dissertation > Political, legal > Legal > UNIVERSITY > Forensic

Study on the Postmortem Redistribution of Morphine in Dogs and Decomposition Kinetics in Preserved Specimen

Author DongZuo
Tutor WangYuZuo
School Shanxi Medical
Course Forensic
Keywords morphine postmortem redistribution decomposition kinetics GC GC/MS dog
CLC D919.8
Type Master's thesis
Year 2010
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Objective To establish the analysis methods of morphine in biological material by GC and GC/MS. To develop the morphine acute poisoning dog model for study the postmortem redistribution and decomposition kinetics of morphine. Study the postmortem redistribution of morphine in dog body and decomposition kinetics of morphine in preserved samples, and to provide a scientific evidence for the forensic identification of heroin or morphine poisoning.Methods 1. After adding SKF525A (Internal Standard, IS) to blood and tissues, the specimen were acid hydrolyzed and alkalized to pH9 by sodium hydroxide, then extracted with trichlormethane-isopropanol(9:1),evaporating organic solvent, derivatized with proprionic anhydride-pyridine(3:2) in microwave thermal power 80 over 3 min. The extract was analyzed qualitatively and quantitatively by GC/MS and GC with an internal standard and working curve method for morphine. The data was processed using statistics software SPSS13.0.2. Twelve dogs was divided into intragastric administration group, intravenous injection group and control group. The dogs were executed after administration of morphine one hour, then the corpse preserved at 20℃.The heart-blood, liver, muscle(chest muscle, right forelimb muscle and left forelimb muscle) and brain were taken at 0h,2h,4h,8h,12h,24h,48h,72h,96h after the death respectively. The specimen were extracted and analyzed by GC/MS and GC equipped with NPD for study the postmortem redistribution of morphine in dogs.3. Five dogs was administered intravenous injection of morphine with a dose of 2 LD50 (LD5o=175 mg/kg). When the dogs were executed after administration of morphine one hour, the heart-blood, liver was taken and divided equally four parts, then preserved at 20℃(1% sodium fluoride solution adding in blood and no sodium fluoride, or 4% formaldehyde in liver),4℃and -70℃(or-20℃). The specimen were taken at 0d,8d,35d and 65day for morphine testing.4. The human corpse blood containing of morphine (400μg/mL) was ultrasound mixing four hours and preserved at 20℃(1% sodium fluoride solution and no sodium fluoride),4℃and-70℃respectively. The specimen taken at 0d,8d,35d and 65d respectively were detected for study the decomposition kinetics of morphine in preserved specimen.Results 1. Analysis methods of morphine:The characteristic ion of morphine derivation were 341,268,397,218(m/z) by GC/MS. The regression equation, linear range, correlation coefficient, recovery rate, limit of detection of morphine by GC were Y=0.0796X+0.1033, 0.2-120μg/mL,0.9962,92.6±0.55(%),0.02μg/mL(S/N>3)in heart blood and Y=0.0457X+0.0656, 0.2-120μg/mL,0.9955,90.07±0.67(%),0.02μg/mL(S/N>3)in liver respectively.2. Postmortem redistribution:Morphine intragastric administration group:When the dog corpse stored at room temperatures, the morphine concentrations in heart-blood, liver, left forelimb muscle and brain rose significantly than that at 0 hour with increasing of dogs death period during 96h(P<0.05). While morphine concentration in the right forelimb muscle and chest muscle no change(P> 0.05).Morphine intravenous injection group:The morphine concentrations in heart-blood, liver, brain, right forelimb muscle and left forelimb muscle were significant higher than that at 0 hour during 96h (P<0.05). While morphine concentration in the chest muscle no change (P>0.05).3. Decomposition kinetics of morphine in preserved specimen:The experimental results showed that morphine content decreased gradually both in blood of preservative morphine intoxation dog and in human corpse blood containing of morphine when stored at 20℃,4℃,-70℃,20℃(1%NaF). While the morphine content in liver no significant change when stored at 20℃,4℃,-20℃,20℃(4%formaldehyde).Conclusion 1. The GC/MS and GC methods established for detecting morphine is rapid,sensitive,accurate and reproduction quality which could meet the requirement of our subsequent research,clinical diagnosis and drug poisoning (death) cases.2. Established animal model of postmortem redistribution of morphine and decomposition kinetics in preserved specimen can be used to study the forensic toxicokinetics of morphine.3. Morphine distributed extensively in the tissues, brain and blood of dog. The postmortem redistribution of morphine really happened in dogs. During the dog bodies poisoned with morphine were deposited during 96h, the heart-blood, liver, brain and left forelimb muscle are making higher morphine content in dogs at intravenous injection group and intragastric administration group. In intravenous injection morphine group, morphine content is significant higher in right forelimb muscles after dogs death. The administration mode and corpse body posture can effect the postmortem redistribution of morphine in dogs.4. Morphine in blood easy to be decomposed when stored at 20℃,4℃,-70℃and 20℃(1% NaF), while morphine in liver is relative stable compare with in blood. The preserve temperature and antiseptic don’t effect the content of morphine whether in blood or liver.

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