Effect of Cysteamine on Peripheral Cell-Mediated Immune after Operation of Fistule Installation
|School||Nanjing Agricultural College|
|Course||Basic Veterinary Science|
|Keywords||operation amount of lymphocyte transformation of T lymphocyte cysteamine IGF-1|
In order to investage the effect of cysteamine on cell-mediated immunity and inflammation, the following experiments were carried out:I Effecr of cysteamin on peripheral immune indexes in acute-phase of post-operation periodPart1 Effecr of cysteamin on proliferation of peripheral blood T lymphocyte in acute-phase of post-operation period8 local goats fed with peanut stems,were randomly divided into 2 groups:control and experiment groups. Every goat experienced operation of fixing permanent fistule on rumen and duodenum respectively.Concentration (300g/goat-d) was added to the diet of all goats. Cysteamine(CT2000,45mg/kg·BW·d) added only to the diet of experiment group 3 days before operation on rumen and lasted until the end of the whole experiment. The fresh blood were collected on the day before operation, lth,2th,3th,4 th,8th day after operation on rumen,also before, on the lth,2th,3th,4th,8th day after operation on duodenum.The samples were used to test the transformation of peripheral blood lymphocyte(TLC)in 30 minutes. The results was that on the first day after the two operations,TLC decreased 39.7%和31.15% in the control (p<0.05),showed the depression of operation on proliferation function of peripheral blood lymphocyte. while there was not obvious change in experiment group yet had the tendency to increase. Between the groups,TLC was significantly higher in experiment group than in control on the 2th(61.16%),3th (67.03%),4th(80.07%),8th (111.94%)day after ruminal operation(p<0.05), and also higher before duodenum operation(118.03%), on the 1th(75.53%),8th(60.07%) day after duodenum operation(p<0.05),which demonstrated that cysteamine improved TLC after operation and alleviated the cell immunesuppression.Part 2 Effect of cysteamine and IGF-1 on transformation of peripheral T lymphocyte in vitro Lymphocytes were separated from peripheral blood of 6 goats and cultured.There were three groups:control,cysteamine(38.58μg/ml)and IGF-1(25ng/ml).Compared with the control group, cysteamineand IGF-1 significantly inceased the TLC (158.07%,46.42%; p<0.05),which indicated that cysteamine and IGF-1 can stimulate the TLC directly.Part 3 Effecr of cysteamin on the account of lymphocyte and some inflammation index in acute-phase of goats6 local goats fed with peanut stems,were randomly divided into 2 groups:control and experiment groups. Every goat experienced operation of fixing permanent fistule on rumen and duodenum respectively.Concentration (300g/goat·d)was added to the diet of all goats.And Cysteamine(45mg/kg·BW·d)added only to the diet of experiment group just on the day of operation on rumen and ended on the 8th day after operation. There was 14 days to recoverd before operation on duodenum. And the feeding was completely same as ruminal operation. The fresh blood were collected on the day before operation, 1th,4 th,8th day after operation on rumen,also before, on the lth,2th,3th,4th,8th day after operation on duodenum in order to detected the account of leucocytes, lymphocytes, neutrophils and the respiratory burst. Serum was separated to detect the content of IL-1β,IL-8 and TNF-α.After operation the account of lymphocyte diminished sharply in control group(83.53%,p<0.05),showed the depression of operation on the amount of lymphocyte. There was a smaller change in experiment group(47.87%,p<0.05). Lymphocytes in experiment group were much more than control on the 1st (188.23%),8th(50%) day after operation on rumen and on the 1st (56.51%)day after on duodenum (p<0.05). An increase of leucocytes(113.9%), neutrophils (264.70%) and cytokine IL-1β,IL-8 were observed. There was no apparent effect of cysteamine on cytokine IL-1β,IL-8 and TNF-α. Leucocytes and neutrophils were more in experiment group than in control after operation on duodenum.And also there was no difference of respiratory burst between two groups.The results indicated a positive effect of cysteamine on the accountof lymphcytes,releasing the depression of the operation.There was a weak proinflammation of cysteamine also.Summary:The operation surpressed the amount of lymphocyte and the transformation of T lymphocyte.Cysteamine promoted proliferation of T lymphocyte directly and increase the amount of lymphocyte,or may be through IGF-1.There was no influence of cysteamine on inflammatory factors. And only effect the amount of WBC and neutrophil.ⅡInvestigation of immuine cell in forestomach of goatsPart1:Investigation of immuine cell in rumen and omasum mucosaWe slaughtered and observed the rumen and omasum mucosal after HE-staining.The result was there was many lymphcytes and neutrophils in rumen and omasum mucosal.Most of them were observed in lamina propria,and few in epithelium.Part2:Change of the rumen mucosal surrounding the syrinx10 young goats were slaughtered after the installation for 2 months. After HE-staining,we observed the change of rumen mucosal.The papillae were rare and stunting. And extensive hyperplasia, hyperplastic downward growth,prolongation and numerous epithelial islands were found in the lamina propria.