Dissertation
Dissertation > Agricultural Sciences > Crop > Economic crops > Oil crops > Soybean

Physiological and Cellular Mechanisms of Salt Tolerance Improvement in Glycine Max with Salt-born Glycine Soja

Author ZhangXiaoKe
Tutor ZuoBingJun
School Nanjing Agricultural College
Course Botany
Keywords Wild soybean Cultivated soybean Hybrids Salt Tolerance Physiological indicators SIET Protoplasm
CLC S565.1
Type Master's thesis
Year 2009
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Seedlings cultivated and shoals wild soybean crosses (N23674 × BB52) parents and their salt tolerance screening by each generation hybrids obtained in 4076 (F5) material, first soybean roots and leaves, explore soybean protoplast isolation and purification methods and its influencing factors; processing 0,2,4,6 d and 140 mmol L-1 NaCl solution, from the accumulation of dry matter, leaf area, root absorption area, membrane permeability and osmotic adjustment substances and physiological ion content changes, and SIET (non-injury microbolometer technology) a comparative study of its salt tolerance characteristics from the cellular level. Main results are as follows: suitable types of soybean root and leaf protoplasts isolated enzyme concentrations were CPW-13M {CPW (cell cleaning solution) 13% (w / v) mannitol} 3% cellulose (cellulose R-10) 1.1% pectinase (macerozymeR-10) 1.0% hemicellulose The enzyme (Hemicellulase) and 0.15? Cl2.2H20 9% mannitol? llulase R-10 0.20% pectolaseY-23, pH 5.8, reaction temperature to 28 ° C . Root hydrolysis time 16 h, the protoplast yield up to 1.46 x 105 / g FW, the vitality of 57.8%; leaves hydrolysis time of 4 h, the the protoplast yield up to 1.74 x 106 / g FW activity reached 70.3%. Root yield and vigor two considerations, the protoplasts with 23% sucrose and CPW-18M mixed after sinking purified better, the leaves float purification better with 25% sucrose. 's 1/2 Hoagland solution containing 140mmol · L-1 NaCl treatment 0,2,4,6 tested material germination and seedling root, leaf damage of N23674 gt; 4076 gt; BB52; root absorption area and the order of the size of the root activity for 4076 gt; BB52 gt; N23674. With the increase in the time of salt stress, the decline in the test material roots, leaves and chloroplasts center content, Na, Cl-content and Na / K, a BB52 and 4076 body Na Cl-content increase or decrease in K content was less than N23674, But contrary to the Na / K case. Compared with Na, BB52 and 4076 roots of K absorption and transport to the leaves selectivity (SK, Na) is strong. 3 materials chlorophyll content, net photosynthetic rate and PS I Ⅱ maximum photochemical efficiency decreased gradually N23674 larger decline. Under Salt Stress soybean chloroplasts Na, Cl content and Na / K and net photosynthetic rate was highly significant negative correlation, the N23674 is particularly obvious. 140 mM NaCl stress, compared with the cultivated soybean N23674 wild soybean the BB52 populations and the hybrids 4076 in terms of root tissue or in root and leaf protoplasts showed stronger ability of Na and Cl-efflux as well as on H, K, Ca2 relatively stable internal flow capacity, particularly in protoplasts, which its highly tolerant to salt is consistent with the above, through the cultivation and the shoals wild soybean hybridization and multiple generations of selection, the offspring 4076 (F5) salt tolerance can be significantly improved.

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