Qiangjing Capsule Impact on Epididymis Structure and Function of Varicocele Rats
|School||Chengdu University of Traditional Chinese Medicine|
|Course||Surgery of Chinese Medicine|
|Keywords||Strong Capsules Varicocele Infertility Sperm quality Epididymis structure α-glycosidase L-carnitine|
Objective: To observe the strong Capsules varicocele rats epididymal structure and function of their sperm quality , and to explore the possible mechanism of the compound Chinese medicine model rat epididymal sperm maturation microenvironment regulation . Methods : 100 adolescent male SD rats , 10 were randomly selected for the sham group , the remaining 90 to narrow the left renal vein to establish a rat model of varicocele . 4 weeks after confirmed 72 rats modeling , were randomly divided into model group , strong Capsules , in the low-dose group , five the capsule Yanzong group and Shaofuzhuyu capsule group , n = 12 . Strong fine capsules were given saline and different concentrations of Yanzong capsule , Shaofuzhuyu capsule suspension 10ml/kg gavage once daily for 4 weeks , observed changes in rat epididymal sperm density and vitality observe pathological changes of the epididymis , to detect the content of the epididymis α-glucosidase enzyme ( glucose oxidase ) and L- carnitine content (DTNB method) . The experimental data are processed using SPSS16.0 software . Results: The model rats epididymis low sperm motility and density of alpha - glucosidase and L- carnitine content is low ; strong Capsules motility and density of the high-dose group , α - glucosidase and L- carnitine content in low- dose group , five the capsule Yanzong group , the Shaofuzhuyu capsule group was significantly higher (P lt; 0.01); sperm motility and density of strong fine capsules in the dose group , low levels of α - glucosidase and L- carnitine dose group , Shaofuzhuyu capsule group was significantly higher (P lt; 0.01, P lt; 0.05), but compared with Yanzong capsule group difference was not obvious ( P gt ; 0.05) . Light microscope to find the model group rat epididymal interstitial edema , vascular congestion expansion obvious inflammatory infiltration , the epididymal tubular atrophy , epithelial degeneration , cell disorder ; varying degrees of improvement in each treatment group , high dose group of strong Capsules The improvement is most obvious . Conclusion : strong Capsules can improve the model rat sperm density and vitality , improve the epididymal alpha - glucosidase and L- carnitine content , improve the epididymis morphology , and may be achieved by the above model the rat epididymis micro environment improvement improve fertility purposes .