EA Stomach Meridian related signaling molecules on gastric mucosal injury in rat serum protein expression

Objective: by observing the Yangming Meridian on acute gastric mucosal injury in rats gastric mucosal injury index, morphology and protein expression of signaling molecules in the serum, from the point of view of proteomics EA Meridian mucosal damage mechanism, in order to better understand the mechanism of acupuncture treatment as well as the stomach and the stomach relevance for clinical applications of acupuncture certain experimental and theoretical basis for the method: The study is divided into two parts, the first part: 40 male SD rats were randomly divided into four groups, the blank group, model group, acupuncture group, acupuncture control group, n = 10. Anhydrous ethanol gavage method of preparation of gastric mucosal injury in rats model, EA 7 days, Guth method for gastric mucosal injury index count, HE staining morphological changes of gastric mucosa, the second part: 20 male SD rats were randomly divided into four groups, the control group, model group, acupuncture group, acupuncture control group, 5 / group. Anhydrous ethanol gavage prepared gastric mucosal injury rat model by SELDI technology of electro-acupuncture Meridian (hole), the gastric mucosal injury in the serum protein expression of repair-related signaling molecules. Results: 1. Gastric mucosal injury index (UI) values ??of the model group was significantly higher than the blank group (P lt; 0.01), successful modeling UI value of the EA group was significantly lower than the model group and the power for the control group (P lt ; 0.01), while no significant difference compared with the control group. Observed by light microscopy results consistent with. 2 compared with the blank group, model group and acupuncture control group, serum protein expression profiles of the relevant signaling molecules, electro-acupuncture treatment serum protein expression increased expression of three marker proteins, with a significant difference (P LT ; 0.05) mass-to-charge ratio are 4474.775Da, 15231.14Da, 13826.55Da; 3. compared with the control group, model group and acupuncture control group, serum protein expression related signaling molecules, electro-acupuncture treatment group, serum protein expression in four signs proteins were decreased with a significant difference (P lt; 0.05), their mass-to-charge ratio is 4268.035 Da, 17352.76 Da 13071.32Da 15216.11Da. Conclusion: EA \the stomach relative specificity Contact; Yangming stomach Meridian specific changes related signaling molecules in the serum protein expression, and was a two-way, these expression changes may be due to Yangming Stomach Meridian points, so as to achieve the protective effect on the gastric mucosa. From the molecular level confirmed the relatively specific contact Meridian stomach, and provide an experimental basis for further research.