Dissertation > Medicine, health > Preventive Medicine,Health > Health - basic science > Health Toxicology

Study on the Interaction of Nipagin Ester Preservative with Human Serum Albumin

Author GuoLi
Tutor ChenHongLi
School Lanzhou University
Course Analytical Chemistry
Keywords Human serum albumin Preservative Nebo gold esters Interaction of Fluorescence spectra Circular dichroism UV spectra Binding of Molecular shape Fluorescence titration
CLC R114
Type Master's thesis
Year 2009
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Nipagin ester as a kind of important preservatives has been widely used in food, beverage, cosmetic, medicine and many other areas. The use of preservative in food has many advantages, such as simple method, less investment in equipment, and so on. But it is necessary to limit the dosage of preservative from economic, toxic and other considerations. Human serum albumin (HSA) has a high concentration in blood plasma and has many physiological functions, it can realize transport and distribution of many small molecules and metabolites in the plasma. Preservative-HSA interaction should have a great influence upon the distribution of the preservative in the body, upon their patterns of metabolism and excretion, investigating the binding mechanism of preservative binding to HSA can provide an importance theoretical base on the scientific and reasonable use of preservative. On the basis of the previous researches, the following major innovative works were carried out in this dissertation:(1) For the first time, the interaction between nipagin esters preservative (propylparaben) and human serum albumin was investigated;(2) The effects of propylparaben on HSA second structure were investigated by fluorescence spectroscopy, ultraviolet spectrum, FT-IR and circular dichroism techniques. The computational modeling method was used to study the interaction between propylparaben and HSA, and the results were in consistent with the experimental results.This dissertation consists of three chapters:Chapter 1: The structures, functions and natures of HSA were briefly introduced. The methods、types of interaction force and evolvement about the interaction of different kind of small ligands with protein were reviewed in detail. Chapter 2: The binding of propylparaben to HSA has been investigated for the first time by the fluorescence method, ultraviolet spectrum, FT-TR, CD spectroscopy and molecular modeling. The results have clearly indicated that propylparaben can bind tightly to HSA with changing slightly its molecular conformation. Meanwhile, from the results of binding mode and molecular modeling, it can be revealed that propylparaben has bound within the subdomain IIA of HSA mainly by the hydrogen bond.Chapter 3: The HCuNVAsPWO heteropoly compounds catalysts were prepared by Combination method and characterized by IR spectrum technique. The effects of the contents of Cu and As and the calcining temperature on the activity of catalysts were studied. The resuhs show that the additions of Cu and As are very important for increasing the activity and the yield of the acetaldehyde. The catalysts calcined at 350℃have the highest activity.

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