Dissertation > Medicine, health > Preventive Medicine,Health > Environmental health,environmental medicine > Water and water supply sanitation > Health surveillance of the health standards of water and water

Distribution of antibiotic resistance genes and the evolution of the resistance gene in the Haihe River Basin

Author ZhangHongJie
Tutor MaoDaQing
School Shenyang Pharmaceutical University
Course Microbial and Biochemical Pharmacy
Keywords Haihe River Basin Antibiotic resistance genes Quantitative PCR Evolutionary analysis
CLC R123.1
Type Master's thesis
Year 2009
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In the first of the seven major river systems in the Haihe River Basin and coastal pollution , and pollution has a direct impact on the Bohai Sea water quality . Which pollution has a very serious abuse of antibiotics lead to antibiotic resistance genes . Therefore it is necessary to the Haihe River Basin as a typical area , carried out for the study of antibiotic resistance of the base of the Haihe River Basin and coastal pollution . Firstly, through the ordinary PCR detection of environmental samples , including seven kinds of antibiotic resistance genes in water samples and sediment samples , respectively, for the tetracycline resistance gene : tetT , tetO tetQ tetM and tetB gene ; sulfa resistance gene : sul Ⅰ I sul Ⅱ. The detected samples containing the resistance gene , using real-time quantitative PCR instrument quantitative detection purposes resistance gene . The experimental results show that the sulfa resistance gene sul Ⅰ and sul Ⅱ of the highest detection rate in all samples , and the detection rate of 100% , the resistance gene detection in Xinkai , Hung Nihe , and happiness of the river in the type and copy number on are generally higher than in other regions , three places by the antibiotic resistance genes is more polluted . Entrez gene data search a variety of antibiotic resistance genes . These resistance genes , using the nearest neighbor method , the minimum the evolutionary fat and maximum parsimony constructed phylogenetic tree of antibiotic resistance genes and evolutionary analysis of these resistance genes . Found that similar protein coding function or have a similar mechanism of resistance mediated by the resistance of the different types of antibiotic resistance genes in the evolutionary tree of evolutionary relationships are closer .

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