TGF-β1 Polymorphisms and Risk of Parkinson’s Disease in a Han Chinese Population.
|Keywords||Parkinson’s disease Transforming Growth Factor-β1 Genetic polymorphism|
Objective Parkinson’s disease(PD) is characterised by a progressive degeneration of dopaminergic neurons in the substantia nigra. Recent studies revealed that inflammatory processes contribute to the neuronal degeneration and genetically determined differences in the immune response. There is a hypothesis that Transfom growth factorβ1 (TGF-β1), as a member of pro-inflammatory cytokines, might influence the risk of sporadic PD occurrence and development. To prove it, two DNA polymorphisms at TGFβ1 promoter,-509C/T (rs1800469) was examined in sporadic PD patients in a Han Chinese Population.Methods Polymerase chain reaction-restriction fragment length polymorphism method(PCR-RFLP) was used to analyze the genotype of 390 sporadic PD patients and 328 controls. And direct sequencing performed in 10% of the samples to validate the genotyping results.Results The distribution of TGFβ1 gene -509C/T polymorphisms was not different between PD group and control group, and there were no significant age-related differences in PD patients compared with every healthy-matched control subgroup(P>0.05). Grouped by gender, the polymorphims distributions were different between male PD group and gender-matched control subgroup(P<0.05) and were no significant differences between femal PD group and every healthy-mathched controls subgroup(P>0.05).Conclusions TGFβ1 gene promoter -509C/T polymorphism is a risk factor of male Parkinson disease patients in Chinese Han population.