Synthetic Extraction and Polishing Process of Total Flavones and Procyanidins from Hawthorn Leaves
|School||Tianjin University of Science and Technology|
|Course||Microbial and Biochemical Pharmacy|
|Keywords||Hawthorn leaves Flavonoids Proanthocyanidins ZTC Ⅱ type clarifier Macroporous resin Extract IR|
Hawthorn Rosaceae genus Shanlihong or hawthorn hawthorn dried ripe fruit. Main function is digestion stomach , qi stasis. In recent years, with hawthorn leaves to treat cardiovascular disease, more research . Its chemical composition is mainly terpenes and flavonoids are three major categories , especially flavonoids more research . This article from hawthorn leaves flavonoids and proanthocyanidins two aspects of the extraction and refining process of systematic study . Hawthorn leaves flavonoids on the water extraction process was explored . Through experiments to determine the optimum extraction conditions were : 1:20 , extraction time was 2h, extraction times is 2 . Using ZTC Ⅱ type of natural adsorption clarifying agent makes NaNO2-Al (NO3) 3 colorimetric determination of total flavonoids improved accuracy , error decreases , can eliminate tannin and flavonoid contents of polysaccharides on the interference with the quantitative determination of flavonoids content get better results and to avoid clogging of the resin . Use of macroporous resin extract of hawthorn leaves flavonoids purified , and resins were screened , the method is simple, the result is ideal. Adsorption - desorption preferably D-101 Resin adsorption - desorption conditions were studied to determine the optimum refining conditions were: 4.54-6.02 pH value of the liquid column ; the column velocity 3BV / h; the sample amount 4BV-5BV; sample concentration 1.25g / L; eluent of 60% ethyl alcohol; eluent flow rate of 2BV / h; as an eluent 1BV. Under these conditions , refined out of the flavonoid purity 89.1% , to meet the hawthorn leaves flavonoids as pharmaceutical bulk drugs must be greater than 80% purity requirements. Experiment optimized extraction conditions hawthorn leaf procyanidins determine the ethanol - water as a solvent when the optimum extraction conditions were: extraction concentration 70% , extraction time 60min, extraction temperature is 50 ℃, solid-liquid ratio of 1 : 6, extraction times of 1 second . Analysis of variance by orthogonal experiments to determine the temperature on hawthorn leaf extract proanthocyanidins have a significant effect . In this condition, the yield was 3.85% .