Dissertation
Dissertation > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes

Effect of rhEPO on the Retinal Neurons in Experimental Diabetic Rats

Author LiJin
Tutor ChenXueYi
School Xinjiang Medical University
Course Ophthalmology
Keywords Diabetic retinopathy ERG Rats Apoptosis Erythropoietin
CLC R587.1
Type Master's thesis
Year 2009
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Objective: To study recombinant human erythropoietin hormone (Recombinat human erythropoietin, rhEPO) provide new strategies for clinical and experimental evidence for the possible protective effect of early diabetic rat retinal neurons . Methods: 45 Wistar rats were randomly divided into three groups , the control group , diabetic model group , and rhEPO intervention group , streptozotocin -induced diabetic rat model ; 2.6 weeks after measuring ERGa wave , b -wave , OPs latency and amplitude to observe the analysis of the retinal light microscopy and electron microscopy sections ; 3 . situ terminal transferase labeling (TUNEL) technique observed retinal neuronal apoptosis . Results : the 1.rhEPO intervention group F-ERGa, b, OPs wavelet Op1 Op2 , Op3 crest latent when compared with the normal group, no significant significance ( P gt ; 0.05) compared with diabetic model group , there were significant ( P lt; 0.05 ) ; the rhEPO intervention group F - ERGa b volatility and OPs total volatility diabetes model group was significant ( P lt; 0.05 ) ; light microscopy , electron microscopy : early diabetic rat retinal nerve ganglion cell layer , inner granular layer structural abnormalities of the retina of diabetic rats of rhEPO intervention group structure near normal ; 3.TUNEL staining : diabetic model group significantly increased apoptosis of rat retinal cells , ganglion cell layer and inner granular layer were seen in a large number of expression of apoptotic cells positive for EPO intervention group retina of diabetic rats apoptotic cells significantly compared to the diabetic group decreased, the difference is statistically significant ( P lt; 0 , 05) . Conclusion : The early diabetic rat retina ERG significant change ; 2 . Retinal neurons undergo apoptosis , diabetic retinopathy retinal ganglion cells and within the external granular layer neuron death one of the important ways ; 3.rhEPO pair apoptosis in early diabetic rat retinal neurons significantly inhibited , and played the role to protect retinal neurons .

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