Dissertation
Dissertation > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease > Cirrhosis

Detection of Serum sICAM-1 and IL-18 in Patients with Hepatitis B Cirrhosis and Their Clinical Significance

Author ZhangXianJun
Tutor HuJianGuo
School Ningxia Medical University
Course Internal Medicine
Keywords Cirrhosis sICAM-1 IL-18 HBV-DNA
CLC R575.2
Type Master's thesis
Year 2009
Downloads 37
Quotes 0
Download Dissertation

Objective To investigate the hepatitis B patients with cirrhosis serum soluble intercellular adhesion molecule -1 (sICAM-1), interleukin -18 (IL-18) and its clinical significance. Methods 60 patients with cirrhosis and 30 patients with normal serum levels of sICAM-1, IL-18 levels were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELIAS). 60 patients with cirrhosis hepatitis B virologic markers were detected by ELISA, fluorescence quantitative PCR detection of HBV-DNA content, automatic biochemical analyzer detection of liver function (ALT, TBIL, ALB), automatic coagulation analysis cytometry prothrombin activity (PTA). Results (1) cirrhosis patients serum sICAM-1 levels significantly higher than the healthy control group (P lt; 0.01), liver cirrhosis the decompensated serum sICAM-1 levels were significantly higher than the decompensated group (P lt; 0.01). Cirrhosis patients with serum IL-18 levels were significantly higher than the healthy control group (P lt; 0.01) the decompensated cirrhosis serum IL-18 levels were significantly higher than those with compensated group (P lt; 0.01) . (2) patients with cirrhosis Child C grade group (C) serum levels of sICAM-1 group (A) was significantly higher than that of Child A and Child B group (B), B group was higher than that in group A, The results showed a significant difference (P lt; 0.01). Hepatitis B patients with cirrhosis group C serum IL-18 levels were significantly higher than in group A than in group A, B, B group, the results showed a significant difference (P lt; 0.01). (3) in patients with liver cirrhosis HBV-DNA positive serum sICAM-1 levels and HBV-DNA negative group compared to no significant difference (P gt; 0.05). Hepatitis B patients with cirrhosis HBV-DNA positive group, serum IL-18 levels and HBV-DNA negative group compared with a significant difference (P lt; 0.01). (4) in patients with cirrhosis serum sICAM-1 levels with PTA, ALB was a significant negative correlation (r = -0.609, -0.373, P lt; 0.01), and TBIL was a significant positive correlation (r = 0.635, P lt ; 0.01), and ALT no correlation (r = 0.004, P gt; 0.05). Hepatitis B patients with cirrhosis serum IL-18 levels PTA, ALB was a significant negative correlation (r = -0.603, -0.392, P lt; 0.01), and the TBIL was significantly positively correlated (r = 0.591, P lt; 0.01) , no correlation (r = 0.148, P gt; 0.05) and ALT. Serum sICAM-1 levels and IL-18 were significantly positively correlated (r = 0.432, P lt; 0.01). Conclusion (1) sICAM-1, IL-18 level of a certain extent reflect the severity of the degree of damage and cirrhosis of the liver cells for clinical estimated condition, prognosis and treatment plan to provide a reference index. (2) sICAM-1 level change has nothing to do with the level of HBV replication, the secondary immune reaction leads to the expression of sICAM-1, sICAM-1 to cause damage to the liver cells. Changes in the level of IL-18 and HBV replication level of activity, clinical determination of the activity of IL-18, and have some practical value in monitoring viral replication status and anti-viral treatment.

Related Dissertations
More Dissertations