Experimental Study on the Effects of Catgut Implantation at Acupoint on Anti-epilepsy and on Hippocampal Amino Acid Neurotransmitter
|School||Hunan University of Traditional Chinese Medicine|
|Course||Acupuncture and Massage|
|Keywords||catgut implantation at acupoint epilepsy epileptic seizures behavior electroencephalogram(EEG) amino acid neurotransmitter hippocampus|
Objective : To investigate anti-epilepsy effect and mechanism of catgut implantation at acupoint, by means of observing the effects of catgut implantation at acupoint on epileptic seizure behaviors, electroencephalogram, hippocampal amino acid neurotransmitter in experimental epilepsy rats.Methods 50 SD male rats were randomized into groups A(normal), B (model), C(treated with catgut implantation at acupoint), D(treated with sodium valproate), E(treated with acupuncture), 10 in each group. Except group A, the rats in the 4 groups received intraperitoneal injection of sodium penicillin to induce acute epilepsy model after 3 days pretreatment. The latent period and the performances of epilepsy seizures within 70 minutes after modeling were observed, the voltage and frequency of EEG in Fz and Pz zones within 70～85 minutes after modeling were detected. After 90-minute modeling, the rats were killed, hippocampus was separated, the contents of gamrna-amino butyric acid (GABA), glutanic acid (Glu), aspartic acid (ASP), alanine (Ala), glycine (Gly) and taurine (Tau) in rats hippocampus were determined, and the ratio of GABA and Glu was calculated.Results:①The latent period of epilepsy seizures of group CD and E was prolongered than group B(P<0.05 or P<0.01), and that of group C was the longest but there was no statistical significance of differences, compared with groups D and E (P>0.05).②The every times of epileptic attack behavior in group C, including nodding / tail-flick, beard shaking / face tic, anterior / posterior limb tic, systemic tic and abruptly jump, were less than those in group B respectively (P<0.05 or P<0.01). The times of systemic tic in group D was obviously less than that in group B (P<0.01), and the times of systemic tic and abruptly jump in group E were obviously less than in group B (P<0.05 or P<0.01). The times of beard shaking / face tic in group C was less than in group D and E respectively (P<0.05 or P<0.01), but the times of abruptly jump in group E was less than in group C (P<0.05).③The degree of epileptic attack in group C was lighter than in group B, but there were no statistical significance in both group D and E compared to group B (P>0.05).④The voltage of Fz and Pz zones in group B increased obviously(P<0.01 compared with group A), and that in group C, D and E decreased (P<0.05 or P<0.01 compared with group B), but there was no statistical significance of differences among the three groups(P>0.05).⑤The frequency A and B of brain wave in group B slowed down(compared with group A), but the frequency A in group C, D and E increased(P<0.05 or P<0.01 compared with group B), the frequency B in group C and E increased (P<0.05 or P<0.01 compared with group B). The effect to frequency A was marked in Pz zone. But the frequencies of A and B in group C, D and E had no statistical significance of differences respectively (P>0.05). ⑥The contents of Glu、ASP in Hippocampus due to excitatory amino acid (EAA) in group B were higher than those in group A(P<0.05 or P<0.01), those in group C, D and E decreased obviously(P<0.05 or P<0.01 compared with group B), but the content of Glu in group C was lower than group D and E respectively (P<0.05 or P<0.01).⑦The contents of GABA、Ala、Gly in Hippocampus due to inhibitory amino acid (IAA) in group B were higher than those in group A(P<0.01), but those of group C increased ( P<0.01 compared with group B and D), GABA and Gly of group C were higher than group E (P<0.01), and Ala of group E increased ( P<0.01 compared with group D). The content of Tau in group B was higher than group A(P<0.01), but that in group C, D and E decreased respectively (P<0.05 or P<0.01 compared with group B) and were near to that of group A (P>0.05) ,but there was no statistical significance of differences respectively among group C, D and E (P>0.05) .⑧The ratio of hippocampal GABA and Glu in group B was higher than that in group A (P<0.01), those in group C and D were higher than group B respectively (P<0.01), and that in group C was higher than group D and E respectively (P<0.05 or P<0.01).Conclusions:①Catgut implantation at acupoint can prolong the latent period of epilepsy seizures, diminish the epileptic attack behavior, lessen the degree of epileptic seizure, thus it has remarkable anti-epilepsy effect, which is better than sodium valproate and acupuncture.②Catgut implantation at acupoint can obviously decrease the brain wave voltage, increase the brain wave frequency, suppress the epilepsy electric discharge, and improve epilepsy EEG, all of which are the same, compared with sodium valproate and acupuncture.③The anti-epilepsy mechanism of Catgut implantation at acupoint may be related to the contents increase of GABA, Ala and Gly due to IAA, to the decrease of Glu and ASP due to EAA, to the stability of Tau, and to the enhancement of the ratio of GABA and Glu in epilepsy rats hippocampus.