Dissertation > Agricultural Sciences > Gardening > Vegetable gardening > Melons > Cucumber

Isolation, Expression, SSR Marker Analysis of Genes Related to Parthenocary of Cucumber

Author WangLei
Tutor ChenJinFeng
School Nanjing Agricultural College
Course Olericulture
Keywords Cucumber Parthenocarpy Aux/IAA ARF Expression SSR
CLC S642.2
Type Master's thesis
Year 2011
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Cucumber(Cucumis sativus L.) is vegetable crops, originated in the south of the Himalayas in northern India. Now widely cultivated throughout the world, it is one of the world’s top ten vegetable crops. Parthenocarpy of cucumber is the target-specific characteristics of new varieties, under the cultivation conditions of the low temperature, low light and no insect, strong parthenocarpic varieties can have a good harvest, Therefore, the further study of parthenocarpy have a great significance on genetic improvement of cucumber. Auxin is the main hormones caused parthenocarpy of cucumber, and other plant growth regulators play an indirect role. Transcriptional regulation of auxin controlled by Aux/IAA proteins and transcription factor ARF (Auxin response factor) family that Aux/ IAA and ARFs can form a hybrid dimmer. with the help of auxin, Aux/IAA is degraded, then auxin signaling pathways opened, causing fruit development.Here, twenty-eight Aux/IAA genes and Eighteen ARF family members were identified from Cucumber Genome Datebase using bioinformatics methods. Then, expression patterns of some Aux/IAA and ARF family members in fruits of cucumber were analyzed by RT-PCR. The results showed that the expression of Csa016715, Csa012805, Csa019264 and Csa010564 only are in the development fruits and higher levels. Thus, it could be speculated that these genes were closely related to fruit development of cucumber.The markers SSR22338 was specific polymorphic in many parthenocarpic plants of F2 populations, and many non-parthenocarpic plants of F2 populations were not the specific polymorphic. This result can be seen the marker SSR22338 is near the parthenocarpic QTL locus.

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