Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice
|School||Nanjing Agricultural College|
|Course||Animal Genetic Breeding and Reproduction|
|Keywords||follicle blood vessel apoptosis angiogenic factor granular cells|
Small preantral follicles have no vascular supply of their own, antral follicles acquire vascular networks in the thecal layer; the vascular capillaries do not penetrate the follicular membrane but they expand during follicular growth. After formation of follicular microvasculature, the preovulatory follicles preferential get nutrition via a more highly developed vascular network.Ovarian follicular angiogenesis is initiated at the early stages of follicular development, and blood vessels in the thecal layer increase in number and size following the development of follicles. These indicate a strong association between angiogenesis and follicular development.To investigate the effects of VEGF-120、FSH、2-ME2、4-OHE2、FSHR antagonist to the development of follicles.Divide the mice into control group and experimental groups (5 mice in each group).Control group is injected PBS, and the experimental groups are injected different chemical agents. After a few days, take one side ovary, make it into continuous slides, and the slides are used to analyze the changes of the different size follicles’number and their blood vessels development by HE staining. Another ovary is used to extract the RNA of granular cells, and analyze the expression changes of VEGF and Flk-1 genes.The result is as follow:(1) FSH and VEGF can increase the ovary weight of the injected sexual maturity mice, while other groups have no dramatic effect to the ovary weight; 2-ME2 may reduce the effect of FSH to the development of ovary, and FSHR antagonist may reduce the effect of VEGF. (2) Various treatments have different impacts on the number of the different size follicles. FSH and VEGF can increase the number of large follicles; The number of medium follicles is increased after the injection of VEGF, however it is decreased after the injection of 2-ME2+FSH and VEGF+FSHR antagonist; VEGF can decrease the number of small follicles, and other groups have no significant effect on the number of small follicles against the control group.(3) Various treatments have different impacts on the percentage of the different size atretic follicles. The percentage of large atretic follicles is increased after the injection of 2-ME2 and4-OHE2, while large atretic follicles are significantly decreased after the injection of FSH and 2-ME2+FSH; The percentage of medium atretic follicles is increased after the injection of 4-OHE2 and VEGF+FSHR antagonist; VEGF can increase the the percentage of small atretic follicles, while other groups have no significant effect.(4) Various treatments have different impacts on the follicular angiogenesis. FSH、VEGF and VEGF+FSH antagonist can significantly increase the number of red blood cells around the large follicles against the control group; The number of red blood cells around the medium follicles is increased after the injection of FSH and VEGF, but decresed after the injection of 2-ME2 and 2-ME2+FSH; 2-ME2 and 2-ME2+FSH can reduce the number of red blood cells around the small follicles, while other groups have no significant effect.(5) The expression of VEGF gene in the granular cells can be dramatic promoted by the injection of VEGF, but significantly inhibited by 4-OHE2, while other groups have no significant effect; The expression of FLK-1 gene can be significantly promoted by the injection of FSH and VEGF.