Qingdao region of hepatitis B virus the BCP gene mutation and hepatitis B virus associated with primary liver cancer
|Keywords||Hepatitis B virus Hepatocellular carcinoma Basic core promoter Gene mutation|
Objective: Hepatocellular carcinoma is one of the most common malignancies worldwide . The annual cases of death due to liver cancer in the world of about 598,000 new cases each year of about 626,000 , the death rate similar incidence . Liver cancer early diagnosis more difficult , once the clinical diagnosis , most advanced , the prognosis is poor , primary liver cancer early diagnosis and early intervention is of great significance . Some studies have shown that the risk of HCC in hepatitis B virus infection is 100 times that of the normal population . Currently , numerous studies have shown that the hepatitis B virus gene mutations and HCC closely related to hepatitis B virus core promoter gene T1753V the double mutant A1762T/G1764A allele of HCC are closely related . We start the sub- gene sequence mutation analysis in Qingdao , Shandong Province, the Han population hepatitis B virus core characteristics , and to explore its correlation with primary liver cancer . Methods: serum samples of patients with chronic hepatitis B and hepatitis B virus associated with primary liver cancer patients receive the 30 cases , and then extracted HBV DNA by polymerase chain reaction (PCR) amplification of the S gene fragment in serum , determined in accordance with the coding sequence of patients genotype and serotype ; core promoter gene fragment amplified HBV DNA and the clones underwent gene sequencing , respectively, of the sequence results with standard strains of HBV in the GeneBank contrast, mutation analysis of the gene sequences DNAMAN software . Statistical analysis was performed using SPSS17.0 software . Results: 60 specimens were amplified S gene , all HBV strains are genotypes B or C , including C-type main the serotypes are adw2 or adrq . In addition to the two cases of chronic hepatitis B patients with serum , and seven cases of liver cancer in patients with serum , the rest were amplified 619bp gene fragment containing the core promoter gene . The common point of the core promoter region of the HBV genome mutations as T1753V the C1653T A1762T/G1764A, incidence were 86.27% ( 44 / 51 ) , 41.18% ( 21 / 51 ) and 45.12% ( 23 / ??51 ) . Compared with patients with chronic hepatitis B , the high incidence in patients with liver cancer mutation sites C1653T (P lt; .001) and A1762T/G1764A ( P lt; .001 ) . Conclusion: Qingdao, Shandong HBV genome is common for B, C genotype , including C-type ; mutations in the core promoter region of the high incidence of , of which , T1753V with HCC unrelated , C1653T , A1762T/G1764A bit point mutation in HCC closely related.