Experiment Research on the Extraction and Separation of Erythromycin
|Keywords||Ionic liquids Erythromycin Extraction and separation Remain|
With the improvement of people's awareness of environmental protection, a growing number of food safety incidents cause of concern, such as milk powder melamine poisoning cases, Red Bull detected cocaine, these events are not only a huge loss to the lives and property of the people, but also brought extremely bad social impact and serious environmental pollution problems. Therefore, how to take effective detection method to detect residual contaminants in food has become a priority. Antibiotic residues in the environment is not only interested in the surrounding environment causing serious pollution, but also cause harm to human health, therefore, there is a need for antibiotic residues in the environment handle. The existing method of detection of antibiotic residues exist low sensitivity range of options than a single, less accurate. Research an analysis fast and easy to operate, accurate method for the detection of trace amount of antibiotics is a priority. Substances in liquid form at room temperature or near room temperature (-30 ° C to 50 ° C), the ionic liquid is completely constituted by the organic cation and an inorganic or organic anion. Makes its study more and more attention because of the unique nature of the ionic liquid. The ionic liquid can be used in many ways, its range of applications including battery electrolytic, the synthetic industrial solvent extraction. In this paper, the two-step synthesis of four different ionic liquids, one-step synthesis of two different hydrophobic ionic liquid, and using UV spectroscopy, nuclear magnetic resonance and infrared spectroscopy to characterize the structure of ionic liquids; to the synthesis of ionic liquids as extractant, extraction of erythromycin, study extracted erythromycin, separation effect, to examine the impact of different factors on the extraction rate, compared ionic liquids and traditional organic solvent extraction rate of erythromycin, The regeneration treatment of the ionic liquid. The best experimental conditions were as follows: [Bmim] PF6: ionic liquid for each 2.00 g, ie 71g/mL temperature of 323.15K the oscillating time for 2.0h pH value of 5, the presence of interfering substances on the extraction rate little effect on the ionic liquid can be reused more than five times; [Omim] BF4: ionic liquid for each 2.50 g ie 89g/mL, temperature 323.15K oscillation time of 1.5h, the pH value of 8, the presence of interfering substances on the extraction rate, the ionic liquid can be reused more than five times; Emim] BF4 / NaH2PO4 double of the water-phase system: the amount of ionic liquid each 3.50g that 125g/mL of solid NaH2PO4 added in an amount of 20.0g, and that the concentration of 39.0%. Temperature of 303.15K, the oscillation time of 2h ~~ 2.5h, the pH value is 6, the ionic liquid can be reused more than five times; [Bmim] BF4 / NaH2PO4 double of the water-phase system: the amount of ionic liquid for each 1.50 g, namely 54g/mL, the amount of solid NaH2PO4 join 11.0g, that concentration of 27.0%. Temperature is 313.15K, the oscillation time of 2.0H, the pH value of 7, the ionic liquid can be reused not more than 5 times. The ionic liquid can be treated further use. The experimental results extracted egg the residual micro Trace erythromycin, and enrichment of ionic liquid, separating the detection method of the environment residual trace amount of erythromycin. The ionic liquids can be enriched in residual trace amounts of erythromycin, and can extracted erythromycin. [Bmim] PF6 linear equations A = 13.6C-0.60, a correlation coefficient of 0.9982, the linear range of 0.5 ~~ 100μg/mL detection limit 4μg/kg, Omim] BF4 linear equation is A = 6.42 C-0.035, a correlation coefficient of 0.9420, the linear range of 0.5 ~~ 100μg/mL detection limit 5μg/kg. Sample erythromycin recoveries were 87.5 to 108.3%. The results showed that the ionic liquid extraction of erythromycin.