The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction
|School||Anhui Medical University,|
|Keywords||myocardial infarction gene polymorphism homocysteine|
Background and Objective Coronary heart disease(CHD) is one of the main disease to threaten human’s health for the time being. In many countries,especially in the developed countries like America and some west European countries,its morbidity rates and mortality rates are increasing quickly. In our country,coronary artery disease has also been taking on obvious ascending trend and has become the leading cause of death in many cities and regions. It was already found that many risk factors countributed to the development of coronary artery disease, such as diabetes mellitus, hypertension,hypercholesterolemia,smoking,gender,age and etc.There are quite a number of“normal”persons with coronary artery disease,who are withlow scores of risk factors of CAD.Besides,there are still many difficulties in correcting or killing the risk factors.Recent studies have showed that hyerhomocysteinemia was also closely related with the development of coronary artery disease.There has been a wealth of epidemiological evidence that there are association between hyperhomocysteinemia and coronary artery disease.This study was designed to investigate the relationship of polymorphisms of methionine synthase reductase (MTRR) gene and plasma homcysteine(Hcy) levels with myocardial infarction.Methods 180 myocaedial infarction patients and 180 coronary artery normal subjects were included in the study. The polymorphisms of their MTRR gene were analyzed using PCR-RFLP and their plasma total Hcy levels were measured using high-performance liquid chromatography and fluorescence detection methods. Results There were three kinds of genotype:GG(homozygous mytation), AG(heterozygous mutation) and AA(wild-type).The frequencies of the three genotypes were as follows:GG, 35.4%;AG, 53.8% and AA, 10.8% in mypcardial indarction patients. Meanwhile, GG, 20.1%;AG,55.8%;AA, 24.1% in coronary artery normal subjects, tespectively. The frequency of G alleles was significantly higher in myocardial infarction patients than in normal controls (62.3% and 32.1%,respectively) (P<0.01). Mean total plasma homoctsteine concentrations were significantly higher in myocardial infarction patients than in the normal subjects(P<0.01). Conclusion These results suggest that polymotphisms of MTRR gene and/or hyperhomocysteinemia may be an independent risk factor for myocardial infarction.