The Influence of Early Intervention of Vitamina Combined with Mycobacterium Bovis Bacillus Calmette-Guerin on Lung CD11C+ and Anti-OVA Special IgE Expression in Adult SD Mice Asthma Model
|School||Anhui Medical University,|
|Keywords||Asthma Vitamin A Mycobacterium bovis bacillus Calmette-Guerin early intervention bronchial asthma CD11C+ anti-OVA sIgE antibody|
Objectives we want to explore the association between early intervention of vitamins A combined with Mycobacterium bovis bacillus Calmette-Guerin and lung pathological change and CD11C+ expression and anti-OVA sIgE concerntration of lung and spleen tissue and serum in adult SD mice asthma model,as to explore how they affect the pathway to asthma immune response and to provide the theory basis for vitamin A and Mycobacterium bovis bacillus Calmette-Guerin use in the prevention and treatment of bronchial asthma.Methods 55 young SD mice at 7days after birth were randomely divided into 4 groups:Group A with 15 rats for the vitamins A combined with Mycobacterium bovis bacillus Calmette-Guerin.Group B for the Mycobacterium bovis bacillus Calmette-Guerin group,Group C for asthma contral group,and with each group 15 rats. 10 rats left belong to the normal control group.On the 1st and 7th day of this experiment,Rats in Group A were given oral vitamin A with dose of 8μL and intraperitoneal injection of BCG with dose of 1μg each time, while Group B received intraperitoneal injection of BCG with each dose of 1μg alone,and Group C had . intraperitoneal injection of NS.All three groups mice were sensitized first time by intraperitoneal injection of 1.0 ml mixture of ovalbumin emulsified in Al(OH)3 on the 35th day of the experiment.And on the 49th day of this experiment,all three group animals received airway challenge of 1% ovalbumin diluted in PBS delivered by aerosolization for 7days and 30minutes ech day.After the 7th airway challenge,the animals were killed,and part of the mid-left lung was made frozen section for the immunohistochemistry of CD11C+,blood was centrifugaled for serum sample,and part of the lung and spleen sample were homogenized frozen until analysis. of anti-OVA sIgE by ELISA,pathological change of each group was also observed.Detection CD11C+ was detected in lung frozen section by immunohistochemistry and concentration of anti-OVA sIgE in homogenized sample of lung and spleen was measured by ELISA,also pathological change of each group was also observed.Results (1) Pathological change comparision between each group:Compared to the lung pathological change of normal control group, group C was not severe as to group B and group C,and it recoverd more quickly. Compared with the normal control group, C group asthmatic rats had more severe lung inflammation :epithelial goblet cells and basal cells increased significantly, airway spasm was surrounded by severe interstitial infiltration of lymphocytes . Significantly widened alveolar septum, alveolar space with plenty of fluids and cell exudation; compared with C group ,rats in B group had airway epithelial goblet cells increased in size ,basal reduce airway spasm is not obvious, there are serous and lumen Exudate cells, peritubular interstitial lymphocytic infiltration, widened alveolar septum, with lymphocyte infiltration, lung inflammation, the pathological changes was reduced . Rats of A group underlying basal cells in the lungs and air lane no goblet cells Change, no airway spasm, no serous and cellular alveolar exudate, slightly widened alveolar septum, septum infiltrating lymphocytes, pathological changes of lung inflammation compared with C group.(2)CD11C+ expression of immunohistochemistry on lung tissue results: CD11C+ expreesion on lung section of D group could be scarely found.CD11C+ expreesion on lung section of A group was significantly higher than those of Mycobacterium bovis bacillus Calmette-Guerin group and asthma C group on the 56th day of immunization intervention,also CD11C+ expreesion of B group was significantly higher than C group.statistically significant can be considered for the P value was of<0.05, (3) anti-OVA sIgE antibody concerntraion results: on the 56th day of early intervention,anti-OVA sIgE level with serum and homogenized lung and spleen sample of the Vitamin A and Mycobacterium bovis bacillus Calmette-Guerin group was significantly lower than those of Mycobacterium bovis bacillus Calmette-Guerin group and asthma C group,also anti-OVA level of the BCG group was lower than asthma C group,but all three group had the lower anti-OVA concentration than that of the contral group.Significant difference could be seen among three groups..Conclusions (1) Erly intervention of vitamin A combined with Mycobacterium bovis bacillus Calmette-Guerin BCG. could reduce the pahological change of adult stham rats . (2) Early intervention of vitamin A combined with Mycobacterium bovis bacillus Calmette-Guerin BCG. could increase the expression of CD11C+ of lung tissue in adult SD mice asthma model. (3) Early intervention of vitamin A combined with Mycobacterium bovis bacillus Calmette-Guerin BCG. could reduce the concentration of anti-OVA sIgE concentration of the serum and lung and spleen tissue in adult SD mice asthma model.