Dissertation > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Heart

Tissue-engineered Construction and Preliminary Implantation Study of Diamond-like Carbon Films for Artificial Mechanical Valve Applications

Author LiDaGang
Tutor YanZhongYa
School Anhui Medical University,
Course Surgery
Keywords Diamond-like Carbon Tissue engineered canines endothelial cells biocompatibility mechanical valve in vitro nitric oxide
CLC R654.2
Type Master's thesis
Year 2011
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Background: Heart valve disease is the most common form of heart disease. Heart valve replacement surgery is performed on 25 million people each year in the world., At least a million people need heart valve replacement surgery in China each year, estimated by Chinese experts Clinical standards. The whole low-temperature isotropic carbon bileaflet is used the most widely in clinical, but it needs life-long anticoagulation after implantation and inappropriate anticoagulation could easily lead to bleeding, thrombosis and embolism in patients, which would impact on the quality of their life seriously,so it’s limited application. With the development of tissue engineering, it is possible to build a new generation of high-quality valve. But suitable biodegradable material valve construction materials has not been found by now, Both of the pulsed laser deposited DLC films and the low-temperature isotropic carbon crystal are non-Carbon materials, but more excellent physical properties and biocompatibility is proved in DLC, which becomes one of optional materials for constructing tissue engineering heart valve .Objective: To investigate the biocompatibility of pulsed laser (PLD) deposition of DLC valve with the canine vein endothelial cells culture and use the method of CVEC with DLC to build completely endothelialization of tissue-engineered heart valve in vitro . we can evaluate blood and tissue compatibility with implantation of TEHV In vivo.Methods: 8 Canines, 6 months old with healthy growth , weighing 10 ~ 15Kg, were numbered from 1 to 8 . Forelimbs saphenous vein endothelial cells were cultured primarily after anesthesia, antigen-antibody reaction associated withⅧwas used to verify cell-derived cells by immunofluorescence. The cells were spread to 2 or 3 generations alternate as seed cells in vitro.16 PLD deposition of the DLC valve is divided into 8 groups, and numbered from A to P. The seed which cultured by each train Canines planted in a group of valve and control group was set up.,which were observed by inverted microscope. NO concentrations of cell culture supernatant were compared on the first ,third ,5th and 7th day. A DLC valve materials in each group was scanned by electron microscopy on the 5th and 9th day and the remaining DLC valve were planted into the corresponding experimental cnine s after completely endothelialized, breeding cnines were killed after 8 weeks. The growth DLC valve cells were observed by SEM and endocardial close to DLC valve was compared to normal endocardia by HE stain.Results: The source of experiments cells were verified endothelial cells byⅧfactor related antigen antibody, canine saphenous vein endothelial cells can culture in vitro, which can be used as seed cells for tissue engineering building. There was no significant difference between the two DLC valve group and control group in the NO concentration in secretion (P> 0.05), cells adhesion to closely to DLC valve scanned by SEM on the 5th and 9th day, the cell pseudopod was extensed and connected to each other. a large number of endothelial-like cells fused on DLC valve after implant in vivo and observed by SEM. There was no significant difference between the two groups in endocardial by HE stain.Conclusion: Primary cultures of canine venous endothelial cells can be used as seed cells in study of tissue engineered heart valve, which showed good biocompatibility with DLC valve deposited by PLD in vitro. a complete endothelialization of tissue Engineered heart valve could be built, which also showed a good blood and tissue compatibility after implantation

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