Isolation and Identification of Oil-degrading Bacteria from the Oil-contaminated Soil and Study on Biodegration of Oil Contaminants
|School||Northeast Forestry University|
|Keywords||Oil pollution Microbial remediation Identification of bacteria|
With the development of industrialization, a growing problem of oil pollution of the soil, the oil contaminants processing has become a world environmental problems to be solved. Bioremediation has efficient, safe, low cost, no secondary pollution and many other advantages to become a major petroleum-contaminated soil treatment in recent years. Which indigenous microorganisms to strengthen the repair method is to become a research focus in recent years. Separation of microorganisms from the start by the shake-flask culture and the soil solid culture screened efficient oil-degrading strains, its preliminary identification of strains growth and oil degradation efficiency of three related factors, and the impact of degradation study to determine the strains in shake flask culture conditions for optimum growth and oil degradation conditions. By studying the results are as follows: 1, from four oil-contaminated soil samples isolated from six bacteria were named X3, X5, X8, X9, number ② 4 and No. 5 ①. 2, the inorganic salt medium shake flask culture conditions, the experiments carried out for 15 days, X3, X5, X8, X9, number ② 4 and No. 5 ① strain on the degradation rate of the crude oil (10g / L), respectively, 57.37%, 59.09% , 46.94%, 31.33%, 38.51% and 49.28%, far higher than that of the degradation rate (21%). Soil extract liquid medium shake flask culture conditions, the experiment for 7 days, X3, X5, X8, X9, No. 4 2, No. 5 ① strains degradation rate of crude oil (10g / L), respectively 49%, 50.08%, 39.10%, 24.85%, 31.12% and 39%, the treatment effect is more ideal. The the soil solid medium culture conditions, the experiment after 15 days, X3, X5, X8, X9, No. 4 2, No. 5 ① strain rate of degradation of crude oil (1%), respectively, 75%, 72%, 64.34%, 68 %, 64.67% and 59%, although the degradation efficiency slightly different between the various strains, but were at a high level, far higher than the control. 3, physiological and biochemical analysis and 16SrDNA whole sequence analysis, X3 strains initially identified as Pseudomonas sp. (Pseudomonas spp); X5 strains of Pseudomonas aeruginosa (P. aeruginosa genus); X8 strains of Acinetobacter junii (Joan Acinetobacter spp.); the X9 strains the Acinetobacter junii (Joan's Acinetobacter); 4 (2) strain Microbacterium oxydans (micro genus); No. 5 ① strains of Pseudomonas aeruginosa (P. aeruginosa L.). 4, by changing the initial salt concentration of the N-source and P source, the initial detection of each strain to the optimum growth and degradation conditions and found that: for X3, the number ② 4 and 5 for ① strains, in the optimal salt concentration 1%; X5, X8 and X9 strain optimum salt concentration of 3%. X3, X5 and the 5th ① strains for optimum N source NH4NO3; For X8 strain the optimum N source NH4Cl; X9 and 4 ② strains for optimum N source as (NH4) 2S04. The optimal P source for all strains are K2HPO4, 3H2O/KH2PO41: 1. In this study, for the indigenous microbial enhanced bioremediation method provides a new strain resources and information, and of great significance to further strengthening effective local microbial.