Dissertation > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials

Research on Synthesis Mechanism and Vitro Property of the Hydroxyapatite Nanocomposite

Author SunXiaZuo
Tutor HeWen
School Shandong Institute of Light Industry
Course Materials Science
Keywords Nano - hydroxyapatite Yeast cells In vitro performance Drug carrier Biocompatibility
CLC TB383.1
Type Master's thesis
Year 2010
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The structure and function of the nano-bio-materials research topic in recent years, the field of medicine and materials and the development direction. Nano hydroxyapatite (HA) as the main component of the hard tissue of human bones, teeth, etc., has a good biodegradability, biocompatibility and higher drug adsorption amount, is an important inorganic biomedical materials. If you can HA prepared mesoporous materials will have a greater value. Yeast cells at room temperature and atmospheric pressure for the template synthesis of mesoporous structure composite nano HA synthesis conditions were optimized using orthogonal test, and system synthesis mechanism and in vitro performance. The entire reaction process under mild conditions, the process is simple, reproducible product structure. The composite nano HA HA prepared than other methods demonstrate superiority. X-ray diffraction, infrared spectroscopy, transmission electron microscopy to characterize the composite nano-HA powder, yeast cells induce crystal nucleation and growth and to control the grain size, the granularity of nano-HA in 80 ° C after drying in about 10-30 nm and a special multi-stage mesoporous and lattice distortion of the structure. Based on the fermentation of yeast cells, metal ion adsorption, hard and soft acid-base rules, biomineralization nucleation and crystal growth experiment principle analysis the yeast template synthesis method. In vitro degradation experiments composite nano HA HA low water absorption than the blank nano, the former has better chemical stability of the immersion medium, and the HA surface Ca2 PO43-ion exchange and deposition, similar to the formation of new bone . Composite nano-HA showed strong fluorescence properties, and onion cell transfection effect. The surface of the particles with a negative charge, the biological activity of the material can be improved. Cells could be a good composite nano-HA surface adhesion and proliferation of material on cell toxicity and hemolysis, but the tumor cells to excessive intake Ca2 toxicity. Composite nano-HA itself has no antibacterial effect of silver loaded HA can effectively kill Gram-positive Staphylococcus aureus and gram-negative Escherichia coli. The composite nano HA very suitable for use as an excellent antibacterial agent carrier. Composite nano-HA can be developed for both the bioactive material having the function of the drug release function and bone repair. The mesoporous structure is conducive to a controlled release of the drug, the hydroxyapatite hydrophilicity and the hydrophobicity of the amino acid group to make the composite nano-HA having amphipathic properties, not only can be used as a hydrophilic pharmaceutical carrier can solubilization a lot of insoluble drugs, to improve the bioavailability of the drug.

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