Assessment of the Drought Resistance and Genetic Stability in Sugarcane Mediated with DREB Gene
|School||Fujian Agriculture and Forestry University|
|Course||Biochemistry and Molecular Biology|
|Keywords||Sugarcane gene transfer EaDREB2B MDA Membrane permeability Soluble sugar Height injury rates Yield and quality traits Glucose metabolism|
Drought has become a constraint sugar production the most important factor. In 2007, the Ministry of Agriculture, Ecology and Genetic Improvement of Sugarcane Physiology Laboratory through the isolation and cloning of sugarcane relatives of the genus E.arundinaceus DREB genes, and use cloned from Arabidopsis inducible promoter RD29A, build efficient plant expression vector by biolistic way to get six drought DREB gene transfer sugarcane clones. In this study, the transfer DREB gene sugarcane materials, T2 transgenic sugarcane genetic stability, drought resistance, yield and quality traits were evaluated, and the transgenic sugarcane drought resistance physiological basis for analysis, the results showed that: EaDREB2B gene in transgenic sugarcane stable inheritance. Using PCR and RT-PCR molecular biology techniques of six transgenic clones EaDREB2B gene testing, the gene in the T2 transgenic sugarcane were in stable expression, control material is not detected the gene, from the DNA level and gene transcription level EaDREB2B that stable and efficient gene expression can. EaDREB2B gene can effectively improve the drought resistance of sugarcane. On the receptor material FN95-1702 and the six transgenic clones for drought stress, found throughout the stress process, FN95-1702 and transgenic clones height growth rate slower than normal watering plants, which FN95-1702's height of the highest injury rate, while transgenic clones of DR22's injury rate is relatively low height, from the point of view that the morphology of transgenic sugarcane DR22 has good drought tolerance. Throughout the course of drought stress, malondialdehyde transgenic sugarcane rate of change of membrane permeability is lower than the FN95-1702, indicating that transgenic sugarcane under drought stress its membrane lipid peroxidation and membrane damage is relatively small. Simultaneously from three protective enzymes (SOD, POD, CAT) changes of point of view, these three enzymes in transgenic sugarcane, scavenging reactive oxygen species has a positive role in reducing lipid oxidation and membrane damage. From the physiological point of view described transgenic sugarcane has some drought tolerance. Under drought stress in transgenic sugarcane soluble sugar study showed that: in the entire stress process, transgenic sugarcane soluble sugar content in the largest increase DR22 is 1634.764%, the smallest DR27 is 1211.893%, higher than the non-transgenic sugarcane FN95-1702's 1202.496%. Description EaDREB2B genes may affect osmoregulation cane to resist drought conditions outside. This is also the angle of osmotic adjustment described transgenic sugarcane drought resistance. In this study, in order to evaluate the performance of the most drought-resistant genetically superior material DR22 and acceptor material FN95-1702 as the research object, analyzes EaDREB2B gene to a certain extent, affect glucose metabolism changes in three key enzymes, sugar cane into sugar accumulation period, Transfer EaDREB2B cane carbohydrate metabolism genes had an impact, reducing the sugar into the speed of other substances to help sugar accumulation. Meanwhile, the transfer of exogenous gene, enabling early entry cane physiological maturity.